BDE-47胁迫对双齿围沙蚕抗氧化防御系统的影响

doi:10.19303/j.issn.1008-0384.2016.06.002

福建农业学报 ›› 2016, Vol. 31 ›› Issue (6): 560-565.doi: 10.19303/j.issn.1008-0384.2016.06.002

BDE-47胁迫对双齿围沙蚕抗氧化防御系统的影响

宿丽丽, 阎希柱

集美大学水产学院, 福建厦门 361021

收稿日期:2016-02-12 修回日期:2016-04-10 出版日期:2016-06-28 发布日期:2016-06-28
通讯作者: 阎希柱(1965-),男,教授,博士,主要从事水生生物生态毒理学和养殖水域生境修复研究(E-mail:yanxizhu@tom.com) E-mail:yanxizhu@tom.com
作者简介:宿丽丽(1989-),女,硕士生,主要从事分子生物学和海洋生物修复研究(E-mail:1090011725@qq.com)
基金资助:
国家海洋公益性行业科研专项子课题（201205009-4）

Effect of the Antioxidant Dfense Sstem of Perinereis aibuhitensis Exposed toTetrabromodiphenyl Ether

SU Li-li, YAN Xi-zhu

Fisheries College of Jimei University, Xiamen, Fujian 361012, China

Received:2016-02-12 Revised:2016-04-10 Online:2016-06-28 Published:2016-06-28

摘要/Abstract

摘要： 将双齿围沙蚕Perinereis aibuhitensis暴露在BDE-47下进行急性毒性试验，测定96 h-LC₅₀。在此基础上，研究了暴露在0、2、4、8 mg·L^-1的BDE-47污染物下，双齿围沙蚕的谷胱甘肽（GSH）含量、谷胱甘肽过氧化物酶（GPx）活力、超氧化物歧化酶（SOD）活力、过氧化氢酶（CAT）活力以及丙二醛（MDA）含量、谷胱甘肽硫转移酶（GST）活力的变化情况。结果表明BDE-47对双齿围沙蚕的96 h-LC₅₀为31.24 mg·L^-1。在暴露期间，与对照组比较，各处理组的GSH含量、GST活力、CAT活力总体上无显著变化，仅8 mg·L^-1组的GST活力在14 d极显著性升高（P<0.01）。暴露1 d时，4 mg·L^-1处理组GPx活力显著升高（P<0.05），在第7～14 d时，2 mg·L^-1处理组的GPx活性呈上升趋势；在暴露1 d时，4 mg·L^-1、8 mg·L^-1两组的SOD活力显著下降（P<0.05），整体上看，在1～7 d时，SOD活力被诱导升高。14 d时，与对照组的比较，4、8 mg·L^-1处理组的SOD活力分别被显著（P<0.05）和极显著（P<0.01）抑制。在暴露1 d时，MDA含量被诱导升高（P<0.05），且4 mg·L^-1极显著升高（P<0.01）。在暴露4～14 d MDA含量总体上无明显变化，仅第7 d的8 mg·L^-1处理组显著升高（P<0.05）。结论：在双齿围沙蚕抗氧化防御系统中，MDA含量和SOD活力均在胁迫初期有显著变化，因此MDA含量和SOD活力可以作为潜在的生物标志物。

关键词: 双齿围沙蚕, BDE-47, 抗氧化酶, GSH, MDA, 生物标志物

Abstract: Perinereis aibuhitensis is used as biological subject in acute toxicity test, for determining the 96 h-LC₅₀ of tetrabromodiphenyl ether(BDE-47).On this basis, the activities of SOD,CAT,GST,GPx and the contents of GSH, MDA of the antioxidant defense system of Perinereis aibuhitensis exposed to 0,2,4,8 mg·L^-1 of BDE-47 pollutants were determined. The results were as follows:the 96h-LC₅₀ of BDE-47 to Perinereis aibuhitensis was 31.24 mg·L^-1.Compared with the control group, the content of GSH and activities of GST and CAT had basically no significant change, only the GST activity of 8 mg·L^-1 group was significantly higher (P<0.01) on 14 th day.The GPx activity of 4 mg·L^-1 group was significantly higher (P<0.05) on 1 th day, and 2 mg·L^-1 group showed the increasing trend during the 7 th to 14 th day.On 1 th day, the SOD activity of the 4 mg·L^-1and 8 mg·L^-1 groups significantly decreased (P<0.05).Overall, during the period of 1-7 d, SOD activity of the 2 mg·L^-1, 4 mg·L^-1groups were induced to rise. On 14 th day, SOD activity in 4 mg·L^-1, 8 mg·L^-1 groups were significant (P<0.05) and significantly (P<0.01) inhibited respectively. The MDA content of all the treatment groups were induced to increase (P<0.05), and 4 mg·L^-1 group significantly increased (P<0.01) on 1 th day. There was no significant change in the content of MDA of the treatment groups during the exposure period of 4-14 th day, only 8 mg·L^-1 group was significantly higher (P<0.05) on 7 th day.In conclusion:the content of MDA and activity of SOD of the antioxidant defense system of Perinereis aibuhitensis had significant changes at the early exposure stage (P<0.05), which could be used as potential biomarkers.

Key words: Perinereis aibuhitensis, BDE-47, antioxidant enzyme, GSH, MDA, biomarker

中图分类号:

X171

宿丽丽, 阎希柱. BDE-47胁迫对双齿围沙蚕抗氧化防御系统的影响[J]. 福建农业学报, 2016, 31(6): 560-565.

SU Li-li, YAN Xi-zhu. Effect of the Antioxidant Dfense Sstem of Perinereis aibuhitensis Exposed toTetrabromodiphenyl Ether[J]. Fujian Journal of Agricultural Sciences, 2016, 31(6): 560-565.

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BDE-47胁迫对双齿围沙蚕抗氧化防御系统的影响

Effect of the Antioxidant Dfense Sstem of Perinereis aibuhitensis Exposed toTetrabromodiphenyl Ether

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