水培枝条取样法在茶树芽叶转录组测序中的可行性鉴定

doi:10.19303/j.issn.1008-0384.2017.02.010

福建农业学报 ›› 2017, Vol. 32 ›› Issue (2): 155-160.doi: 10.19303/j.issn.1008-0384.2017.02.010

水培枝条取样法在茶树芽叶转录组测序中的可行性鉴定

高香凤, 王让剑, 刘丰静, 李慧玲

福建省农业科学院茶叶研究所, 福建福安 355015

收稿日期:2016-08-25 修回日期:2016-11-18 出版日期:2017-02-28 发布日期:2017-02-28
通讯作者: 王让剑(1982-),男,助理研究员,主要从事茶树种质资源与遗传育种工作(E-mail: rangjian.wang@163.com) E-mail:rangjian.wang@163.com
作者简介:高香凤(1979-),女,助理研究员,主要从事茶树分子生物学研究及科研管理工作(E-mail: xiangfeng.gao@163.com)
基金资助:
福建省科技计划项目——省属公益类科研院所基本科研专项（2014R1012-3）

Hydroponic Culturein Sample Preparation of Tea Shoots for RNA Sequencing

GAO Xiang-feng, WANG Rang-jian, LIU Feng-jing, LI Hui-ling

Tea Institute, Fujian Academy of Agricultural Sciences, Fuan, Fujian 355015, China

Received:2016-08-25 Revised:2016-11-18 Online:2017-02-28 Published:2017-02-28

摘要/Abstract

摘要： 为充分验证水培枝条法取样在茶树芽叶转录组测序中的可行性，将水培枝条取样法与干冰取样法所取样本提取的茶树芽叶总RNA完整性、纯度、浓度、转录组测序文库以及转录组测序结果进行比较，结果表明，水培枝条取样法与干冰取样法所取样本提取的茶树芽叶总RNA完整性均良好，纯度均较高，浓度无显著差异；2种取样方法所取样本提取的总RNA品质均符合转录组测序要求，均能成功获取效果相当的转录组测序文库以及转录组测序结果。试验表明水培枝条取样具有简单、方便的特点，更适合野外取样。

关键词: 茶树, 转录组测序, RNA提取, 水培枝条

Abstract: Valid sampling is essential to obtain reliable transcripts of genetic information for biological studies. For RNA sequencing, the integrity, purity and concentration of total RNA as well as the cDNA libraries and transcriptome sequencingon two specimens of tea shoots sampled by using two methods were compared. One of the methods was hydroponic culture (SM1), and another,dry ice freezing (SM2). The results showed that both of them yielded RNA extractions with high integrity and purity without significant differences on the measured concentrations. They were well satisfied for the subsequent transcriptome sequencing, also capable of obtaining the equivalent cDNA libraries and RNA sequencing. However, SM1 was simpler and more convenient than SM2 in handling and operation, and particularly suitable for field sampling. Aside from being used on tea, SM1 could be applied on other crops in preparing samples for extracting RNA as well.

Key words: tea plant(Camellia sinensis), RNA sequencing, RNA extraction, hydroponic culture

中图分类号:

S571.1

高香凤, 王让剑, 刘丰静, 李慧玲. 水培枝条取样法在茶树芽叶转录组测序中的可行性鉴定[J]. 福建农业学报, 2017, 32(2): 155-160.

GAO Xiang-feng, WANG Rang-jian, LIU Feng-jing, LI Hui-ling. Hydroponic Culturein Sample Preparation of Tea Shoots for RNA Sequencing[J]. Fujian Journal of Agricultural Sciences, 2017, 32(2): 155-160.

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水培枝条取样法在茶树芽叶转录组测序中的可行性鉴定

Hydroponic Culturein Sample Preparation of Tea Shoots for RNA Sequencing

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