福建农业学报 ›› 2017, Vol. 32 ›› Issue (7): 746-750.doi: 10.19303/j.issn.1008-0384.2017.07.011

• 食品科学 • 上一篇    下一篇

核桃楸叶多酚提取工艺的响应面法优化及其抗氧化性分析

彭小进, 王天淼, 孙墨珑   

  1. 东北林业大学理学院, 黑龙江 哈尔滨 150040
  • 收稿日期:2017-03-29 修回日期:2017-05-22 出版日期:2017-07-28 发布日期:2017-07-28
  • 通讯作者: 孙墨珑(1961-),女,博士,教授,研究方向为天然产物化学(E-mail:molongsun@126.com) E-mail:molongsun@126.com
  • 作者简介:彭小进(1990-),男,硕士研究生,研究方向为天然产物化学(E-mail:657957680@qq.com)
  • 基金资助:

    中央高校基本科研业务费专项(2572015BB18)

Process Optimization and Antioxidant Activity of Polyphenols Extracted from Leaves of Juglans mandshurica Maxim

PENG Xiao-jin, WANG Tian-miao, SUN Mo-long   

  1. College of Science, Northeast Forestry University, Harbin, Heilongjiang 150040, China
  • Received:2017-03-29 Revised:2017-05-22 Online:2017-07-28 Published:2017-07-28

摘要: 采用核桃楸叶为原料,超声波辅助提取和紫外分光光度计对其多酚类物质进行含量测定。在单因素试验基础上,通过响应面法优化了核桃楸叶多酚类物质的提取工艺,得出核桃楸叶多酚类物质最佳提取条件为:乙醇体积分数70%,液料比15:1,超声时间30 min,超声温度60℃。核桃楸叶多酚类物质对羟基自由基、亚硝酸根离子、超氧阴离子、DPPH自由基有较强的清除能力,其对4种自由基的清除能力强弱顺序为DPPH自由基 > 超氧阴离子 > 羟基自由基 > 亚硝酸根离子,核桃楸叶多酚类物质对4种自由基的清除能力整体强于化学防腐剂苯甲酸,这表明核桃楸叶多酚类物质具有较强的抗氧化活性,是一种天然的抗氧化剂,为下一步研究多酚类物质的抗氧化性提供了依据。

关键词: 核桃楸叶, 响应面法, 多酚, 抗氧化

Abstract: Polyphenols were extracted from the leaves of Juglans mandshurica Maxim using an ultrasound-assisted process. Polyphenol content was determined by UV spectrophotometry. The extraction was optimized by a response surface experiment to arrive at the conditions including 70% by volume of ethanol as solvent, a solvent:solid ratio of 15:1, and an ultrasonic treatment of 30 min at 60℃. The polyphenol extract showed an excellent capacity in scavenging free radicals and ions in the order of DPPH radical > superoxide anion > hydroxyl radical > nitrite ion. It was more powerful than what the chemical preservative, such as benzoic acid, can provide. Thus, the polyphenols extracted from leaves of J. mandshurica by the current method could be a new natural antioxidant for potential applications.

Key words: leaf of Juglans mandshurica Maxim, response surface methodology, polyphenol, antioxidant

中图分类号: 

  • S792.132