福建农业学报 ›› 2017, Vol. 32 ›› Issue (9): 946-950.doi: 10.19303/j.issn.1008-0384.2017.09.005

• 作物科学 • 上一篇    下一篇

彩色马铃薯茎尖培养及病毒检测技术

许泳清1,2, 李华伟1,2, 纪荣昌1,2, 罗文彬1,2, 李国良1,2, 刘中华1,2, 张鸿1,2, 林赵淼1,2, 邱永祥1,2, 邱思鑫1,2, 汤浩1,2   

  1. 1. 福建省农业科学院作物研究所, 福建 福州 350013;
    2. 农业部南方薯类科学观测实验站, 福建 福州 350013
  • 收稿日期:2017-08-04 修回日期:2017-08-28 出版日期:2017-09-28 发布日期:2017-09-28
  • 作者简介:许泳清(1980-),女,副研究员,主要从事马铃薯组织培养技术研究(E-mail:qingqing0722@126.com)
  • 基金资助:

    福建省科技计划项目——省属公益类科研院所基本科研专项(2015R1026-11)

Stem-tip Tissue Culture and Virus Detection on Plantlets of Color Potato

XU Yong-qing1,2, LI Hua-wei1,2, JI Rong-chang1,2, LUO Wen-bin1,2, LI Guo-liang1,2, LIU Zhong-hua1,2, ZHANG Hong1,2, LIN Zhao-miao1,2, QIU Yong-xiang1,2, QIU Si-xin1,2, TANG Hao1,2   

  1. 1. Institute of Crop Sciences, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
    2. Scientific Observing and Experimental Station of Tuber and Root Crops in South China, Ministry of Agriculture, Fuzhou, Fujian 350013, China
  • Received:2017-08-04 Revised:2017-08-28 Online:2017-09-28 Published:2017-09-28

摘要: 为了探明彩色马铃薯茎尖培养方法,获得彩色马铃薯无毒试管苗,研究了不同质量浓度植物生长调节剂组合的培养基对21份彩色马铃薯后代品系组培苗形成的影响,并用RT-PCR方法对试管苗进行马铃薯主要病毒检测。结果表明,筛选出的组合(MS+NAA0.05 mg·L-1+6-BA0.1 mg·L-1+GA30.2 mg·L-1+泛酸钙0.2 mg·L-1)可作为闽薯1号茎尖诱导分化的最佳培养基,优化的植物生长调节剂组合在不同的彩色品种茎尖分化和植株形成差异较大,试管苗经RT-PCR检测6种马铃薯病毒(PVX、PVY、PVS、PVA、PVM和PSTVd),获得了11份彩色马铃薯脱毒苗,可用于大田生产用试管苗。

关键词: 彩色马铃薯, 茎尖脱毒, RT-PCR, 病毒检测

Abstract: Stem-tips of potato(Solanum tuberosum L.) plants that bear colored tubers were used to produce virus-free plantlets in test tubes. Culture media with hormone combinations in varying concentrations were tested to determine their effects on 21 varieties of plantlets offspring of the potato tissue culture. Meanwhile, RT-PCR was employed to detect any viruses present in the plantlets. The optimum medium for propagating a chosen cultivar, Minshu No.1, was formulated on MS with 0.05 mg·L-1 NAA, 0.1 mg·L-1 6-BA, 0.2 mg·L-1 GA3 and 0.2 mg·L-1 calcium pantothenate. Different hormone combinations led to significantly different stem-tip differentiation and plantlet formation among the potato varieties. A total of 11 test tube cultivars derived from the stem-tip cultures were virus-free without the contamination of PVX, PVS, PVA, PVS, PVM or PSTVd. They could be the candidate plantlets for field planting.

Key words: color potato, virus-free stem-tip, RT-PCR, virus detection

中图分类号: 

  • S532