双孢蘑菇As2796子实体发育转录组测序分析

doi:10.19303/j.issn.1008-0384.2018.03.012

福建农业学报 ›› 2018, Vol. 33 ›› Issue (3): 282-287.doi: 10.19303/j.issn.1008-0384.2018.03.012

• 园艺科学 • 上一篇

双孢蘑菇As2796子实体发育转录组测序分析

施肖堃, 蔡志欣, 郭仲杰, 卢园萍, 陈美元, 廖剑华, 王泽生

福建省农业科学院食用菌研究所/特色食用菌繁育与栽培国家地方联合工程研究中心, 福建福州 350014

收稿日期:2017-12-12 修回日期:2018-02-14 发布日期:2019-03-29
通讯作者: 陈美元(1972-),男,博士,教授级高级工程师,主要从事食用菌遗传育种研究(E-mail:cmy1972@gmail.com) E-mail:cmy1972@gmail.com
作者简介:施肖堃(1984-),男,硕士,助理研究员,主要从事食用菌遗传育种研究(E-mail:39094061@qq.com)
基金资助:
福建省科技计划项目——省属公益类科研院所基本科研专项（2017R1020-4、2015R1020-1）；国家现代农业产业技术体系专项（CARS20）；福建省种业创新与产业化工程项目（fjzycxny2017009）；福建省农业科学院科技创新团队建设项目（STIT2017-1-6）

Transcriptome Sequencing on Fruiting Body of Agaricus bisporus in Developing Stages

SHI Xiao-kun, CAI Zhi-xin, GUO Zhong-jie, LU Yuan-ping, CHEN Mei-yuan, LIAO Jian-hua, WANG Ze-sheng

The Edible Fungi Institute of Fujian Academy of Agricultural Sciences, National and Local Joint Engineering Research Center for Breeding & Cultivation of Featured Edible Fungi, Fuzhou, Fujian 350014, China

Received:2017-12-12 Revised:2018-02-14 Published:2019-03-29

摘要/Abstract

摘要： 对双孢蘑菇As2796子实体发育4个不同阶段样品的转录组进行测序，并与双孢蘑菇参考基因组进行序列比对。共比对到9 207个转录本，发掘新基因393个，其中164个得到功能注释。基于比对结果进行各基因在不同样品中的表达量分析，识别差异表达基因，发现原基期与其他3个不同发育时期有316个共同差异基因。对差异基因进行功能注释和富集分析，可为进一步研究双孢蘑菇子实体生长发育的相关基因奠定基础。

关键词: 双孢蘑菇, 子实体发育, 转录组测序

Abstract: Samples of Agaricus bisporus As2796 fruiting bodies in 4 developmental stages were collected for transcriptome sequencing. After aligned with the reference genome sequence of the mushroom, 9 207 transcripts were identified. Among them, 393 were new genes with 164 being functionally annotated. The expression level of the genes was analyzed to differentiate the samples. The alignments showed an overlapping of 316 differentially expressed genes between the primordium and the 3 other developmental stages. An analysis and function evaluation on these genes were performed for further study on the growth and development of A. bisporus fruiting body.

Key words: Agaricus bisporus, fruiting body development, transcriptome sequencing

中图分类号:

S646.11

施肖堃, 蔡志欣, 郭仲杰, 卢园萍, 陈美元, 廖剑华, 王泽生. 双孢蘑菇As2796子实体发育转录组测序分析[J]. 福建农业学报, 2018, 33(3): 282-287.

SHI Xiao-kun, CAI Zhi-xin, GUO Zhong-jie, LU Yuan-ping, CHEN Mei-yuan, LIAO Jian-hua, WANG Ze-sheng. Transcriptome Sequencing on Fruiting Body of Agaricus bisporus in Developing Stages[J]. Fujian Journal of Agricultural Sciences, 2018, 33(3): 282-287.

参考文献

[1] 王泽生,廖剑华,陈美元,等. 双孢蘑菇遗传育种和产业发展[J]. 食用菌学报,2012, 19(3):1-14.
[2] SONNENBERG A S M, BAARS J J P, GAO W,et al. Developments in breeding of Agaricus bisporus var. bisporus: progress made and technical and legal hurdles to take[J]. Applied Microbiology and Biotechnology, 2017, 101(5): 1819-1829.
[3] 陈美元,廖剑华,李洪荣,等. 20个双孢蘑菇核心种质的重测序初步分析[C]//中国菌物学会年会论文集.2015:59-67.
[4] 陈美元,廖剑华,李洪荣,等. 双孢蘑菇子实体发育差异蛋白质组分析[J]. 菌物学报,2015, 34(6):1153-1164.
[5] KIM D, PERTEA G, TRAPNELL C, et al. TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions[J]. Genome Biology, 2013, 14:R36.
[6] FLOREA L, SONG L, SALZBERG S L. Thousands of exon skipping events differentiate among splicing patterns in sixteen human tissues[J]. F1000 Research, 2013, 2:188.
[7] KASPER D. HANSEN,WU Z J, et al. Sequencing technology does not eliminate biological variability[J]. Nature Biotechnology, 2011, 572-573.
[8] ALTSCHUL S F, MADDEN T L, ZHANG J, et al. Gapped BLAST and PSI BLAST: A New Generation of Protein Database Search Programs[J]. Nucleic Acids Research, 1997, 25(17): 3389-3402.
[9] DENG Y Y, LI J Q, WU S F, et al. Integrated nr Database in Protein Annotation System and Its Localization[J]. Computer Engineering, 2006, 32(5):71-74.
[10] APWEILER R, BAIROCH A, WU CH, et al. UniProt: the universal protein knowledgebase[J]. Nucleic Acids Research, 2004, 32: D115-D119.
[11] ASHBURNER M, BALL C A, BLAKE J A, et al. Gene ontology: tool for the unification of biology[J]. Nature Genetics, 2000, 25(1): 25-29.
[12] TATUSOV R L, GALPERIN M Y, NATALE D A. The COG database: a tool for genome scale analysis of protein functions and evolution[J]. Nucleic Acids Research, 2000, 28(1):33-36.
[13] KOONIN E V, FEDOROVA N D, JACKSON J D, et al. A comprehensive evolutionary classification of proteins encoded in complete eukaryotic genomes[J]. Genome Biology, 2004, 5(2): R7.
[14] KERRIGAN R W, CHALLEN M P, Burton K S. Agaricus bisporus genome sequence: a commentary[J]. Fungal Genetics and Biology, 2013, 55:2-5.
[15] 吴小梅,张昕,李南羿. 双孢蘑菇子实体不同发育时期的转录组分析[J]. 菌物学报, 2017, 36(2): 193-203.
[16] CHEN M Y, LIAO J H, LI H R,et al. iTRAQ-MS/MS proteomic analysis reveals differentially expressed proteins during post-harvest maturation of the white button mushroom Agaricus bisporus[J]. Current Microbiology, 2017, 74(5):641-649.
[17] De GROOT P W, SCHAAP P J, SONNENBERG A S,et al. The Agaricus bisporus hyp A gene encodes a hydrophobin and specifically accumulates in peel tissue of mushroom caps during fruit body development[J]. Journal of Molecular Biology, 1996, 257(5):1008-1018.
[18] De GROOT P W J, ROEVEN R T P, VAN GRIENSVEN LJLD, et al. Different temporal and spatial expression of two hydrophobin-encoding genes of the edible mushroom Agaricus bisporus[J]. Microbiology, 1999, 145(5):1105-1113.
[19] LUGONES L G, BOSSCHER J S, SCHOLTMEYER K,et al. An abundant hydrophobin(ABH1) forms hydrophobic rodlet layers in Agaricus bisporus fruiting bodies[J]. Microbiology, 1996, 142(5):1321-1329.

双孢蘑菇As2796子实体发育转录组测序分析

Transcriptome Sequencing on Fruiting Body of Agaricus bisporus in Developing Stages

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