食源性致病菌快速检测技术及其应用研究进展

doi:10.19303/j.issn.1008-0384.2018.04.019

摘要/Abstract

摘要： 随着食品工业的发展，食品安全成为人们日益关注的公共健康问题。在影响食品安全的因素中，病原微生物是最主要的因素之一。因此，建立食源性致病菌的快速检测技术对确保食品安全和保障人类健康意义重大。传统的食源性致病菌检测方法，如微生物培养法和菌落技术法耗时费力，远不能满足食品安全快速检测的要求。目前已报道多种食源性致病菌的快速检测方法，如免疫学技术、分子生物学技术、生物传感器技术等。本文综述了国内外食源性致病菌的快速检测技术及其应用研究进展，比较分析各项检测技术的特点，为新的食源性致病菌检测技术的开发提供参考。

关键词: 食源性致病菌, 快速检测, 免疫学技术, 分子生物学技术, 生物传感器技术, 代谢学技术

Abstract: With the development of food industry, product safety is becoming an increasingly important public health issue. Among the factors that affect food safety, illnesses caused by pathogenic microorganisms is of paramount concern. To prevent or curtail the undesirable incidence from happening, establishment of rapid detection methodologies on foodborne pathogens is one of the indispensable measures. Conventional bacterial detection methods, such as culture and colony counting, are time consuming, laborious and inadequate to meet the demand of a modern society. In recent years, various rapid detection methods have been developed, which apply the technologies involving immunological, molecular biotechnical and/or bio-sensing approaches. This article reviews the methodologies currently available in China and abroad, and compares the pros and cons of the associated technologies and applications.

Key words: foodborne pathogens, rapid detection, immunological technique, molecular biotechnology, biosensor, metabolomics analysis

中图分类号:

TS207.4

陈秀琴, 黄梅清, 郑敏, 陈少莺. 食源性致病菌快速检测技术及其应用研究进展[J]. 福建农业学报, 2018, 33(4): 438-446.

CHEN Xiu-qin, HUANG Mei-qing, ZHENG Min, CHEN Shao-ying. Advances on Rapid Detection of Foodborne Pathogens and the Application[J]. Fujian Journal of Agricultural Sciences, 2018, 33(4): 438-446.

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