不同植物生长调节剂对复合盐胁迫下黑小麦种子萌发的影响

doi:10.19303/j.issn.1008-0384.2019.03.016

摘要/Abstract

摘要： [目的]探讨α-萘乙酸钠（NAA）、赤霉素（GA₃）和6-苄氨基嘌呤（6-BA）3种植物生长调节剂不同质量浓度对不同程度盐胁迫条件下黑小麦种子萌发的影响，为在盐渍地区种植黑小麦提供可能性和科学依据。[方法]以紫麦一号的种子为试验材料，采用纸床法分别测定不同质量浓度的3种植物生长调节剂对不同程度盐胁迫条件下黑小麦种子萌发的影响。[结果]在无盐胁迫条件下，NAA、GA₃、6-BA质量浓度分别为150 mg·L^-1、GA₃ 200 mg·L^-1和6-BA 5 mg·L^-1时，黑小麦种子的发芽率、发芽势等萌发指标均为最佳；复合盐（NaCl：Na₂SO₄=1：1）溶液浸种显著抑制了黑小麦种子的萌发；用不同质量浓度的植物生长调节剂预处理均可在一定程度上缓解复合盐胁迫对黑小麦种子萌发的抑制作用；在中低浓度（40~80 mmol·L^-1）复合盐胁迫条件下，GA₃ 200 mg·L^-1和6-BA 5 mg·L^-1预处理均能有效缓解复合盐胁迫对种子萌发的影响；随着复合盐浓度的增加，各处理种子的发芽势和发芽率明显下降，当复合盐浓度为120 mmol·L^-1时，只有6-BA 5 mg·L^-1预处理对缓解胁迫有一定作用。[结论]在中低复合盐浓度胁迫条件下，采用GA₃（200 mg·L^-1）或6-BA（5 mg·L^-1）预处理能够缓解盐胁迫对黑小麦种子的伤害，从而显著促进在盐胁迫条件下黑小麦种子的萌发。

关键词: 黑小麦种子, 萌发, 植物生长调节剂, 复合盐, 胁迫

Abstract: [Objective]Effects of 3 plant growth regulators on the germination of black wheat seeds under salt-stress were studied to provide possible relief for the wheat planting in saline-alkali areas.[Method] Using a paper bedding method, 1-N aphthaleneacetic acid (NAA), gibberellic acid (GA₃) or 6-benzylamino-purine (6-BA) was applied on seeds of black wheat (Zimai 1) to observe the germination under varied salt concentrations.[Result]The best rate, potential and other indices on the seed germination were found when 150 mg·L^-1 NAA, 200 mg·L^-1 GA₃ or 5 mg·L^-1 6-BA was used without salt-stress. The presence of NaCl:Na₂SO₄=1:1 significantly inhibited the germination. Addition of the plant growth regulators in different concentrations relieved the inhibitive effect to a certain extent. Both GA₃ at 200 mg·L^-1 and 6-BA at 5 mg·L^-1 significantly alleviated the germination inhibition when medium to low salt-stress (i.e., 40-80 mmol·L^-1) was imposed on the seeds. The germination tendency and rate were significantly decreased with increasing salt-stress. When the salt concentration was raised to 120 mmol·L^-1, only the application of 6-BA 5 mg·L^-1 exhibited an effect on the germination.[Conclusion] The treatment of GA₃ 200 mg·L^-1 or 6-BA 5 mg·L^-1 could protect the black wheat seeds from being permanently harmed by a medium-to-low salt-stress and improve the germination.

Key words: black wheat seed, germination, hormone, compounded salt, stress

中图分类号:

S512.1

王泽琼, 蔡珺, 韩国良, 刘勇. 不同植物生长调节剂对复合盐胁迫下黑小麦种子萌发的影响[J]. 福建农业学报, 2019, 34(3): 358-363.

WANG Ze-qiong, CAI Jun, HAN Guo-liang, LIU Yong. Effects of Plant Growth Regulators on Germination of Black Wheat Seeds under Salt-stress[J]. Fujian Journal of Agricultural Sciences, 2019, 34(3): 358-363.

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