Abstract: In order to optimize the extraction process of polysaccharides loach ultrasonic enzyme samples in 100 W ultrasound first ultrasonic instrument 20 min, and then in the enzymatic test based on a single factor, select the hydrolysis temperature, reaction time and dosage neutral protease from variable polysaccharide extraction rate response, the central composite design, study their interactions on polysaccharide extraction rate. The response surface analysis methods, simulated prediction model quadratic polynomial regression equation and determine the best conditions of the Loach Polysaccharide Extraction Process:enzymatic hydrolysis temperature of 50℃, enzymatic hydrolysis time 6 h, neutral protease amount of 5% in under this condition, the polysaccharide was 5.37%.

Key words: loach, polysaccharides, ultrasonic enzymatic, response surface