铁观音及黄棪半同胞系种质遗传多样性的ISSR分析

doi:10.19303/j.issn.1008-0384.2016.10.011

Abstract

Abstract: Two tea cultivars, Tieguanyin and huangdan, are the core parents used in oolong tea breeding. Many premium oolong tea cultivars have been bred from them. Using ISSR molecular markers, 9 primers with high polymorphism and clearly distinguishable ISSR amplified bands were selected for the amplification of 24 tea varieties (or lines) as well as the determination of the genetic diversity and relationship among the cultivars. A total of 73 bands was generated with 15 ISSR primers. Of which, 47 were polymorphic at a rate of 64.4%, averaging 5.2 bands amplified per primer. An analysis on the genetic similarity of the 24 tea cultivars yielded coefficients ranging from 0.644 to 0.889, with an average of 0.727. It suggested that the genetic bases of the germplasms were limited, and their genetic relationships close. The results would provide valuable information for tea breeding in the future.

Key words: ISSR molecular markers, half-sib tea cultivars of Tieguanyin, half-sib tea cultivars of Huangdan, genetic diversity

CLC Number:

S571.1

CHEN Zhi-dan, WANG An-li, SUN Wei-jiang. Genetic Diversity of Tieguanyin and Huangdan Half-sib Tea Cultivars Determined by Using ISSR Markers[J].Fujian Journal of Agricultural Sciences, 2016, 31(10): 1059-1064.

References

[1] 叶乃兴.我国茶树育种的骨干亲本及其系谱分析[J].中国茶叶,2008,4:11-13.

[2] 杨美寅,李文东,董方平,等.湿地松半同胞家系净光合速率日变异规律研究[J].中国农学通报,2008,24(8):171-176.

[3] 韩创举,杨培华,刘永红,等.油松半胞系家系苗期生长性状遗传分析[J].西北农林科技大学学报:自然科学版,2008,36(5):124-134.

[4] 莫亚文.刺槐优树半同胞子代测定研究[J].甘肃林业科技,2007,32(3):29-32.

[5] 孙明高,赵玉涛,宋绪忠,等.银杏半同胞家系综合评价与苗期选择的研究[J].山东农业大学学报:自然科学版,2001,32(4):518-524.

[6] 余继忠.福鼎大白茶半同胞系和云南大叶茶半同胞系遗传多样性和亲缘关系研究[D].北京:中国农业科学院,2010.

[7] 余继忠,杨亚军,黄海涛,等.利用ISSR标记分析福云(半)同胞系茶树品种(系)的遗传多样性和亲缘关系[J].基因组学与应用生物学,2009,28(2):281-288.

[8] 陈志辉,单睿阳,林郑和,等.利用SSR标记分析福建省选育福云半同胞系茶树品种遗传多样性[J].茶叶学报,2015,56(4):238-243.

[9] 段云裳,姜燕华,王丽鸳,等.我国茶树主要骨干亲本及其衍生品种(系)的SSR分析[J].植物遗传资源学报,2011,12(4):533-538.

[10] 王让剑,杨军,孔祥瑞,等.福建15个茶树品种SSR遗传差异分析与指纹图谱建立[J].福建农业学报,2014,29(10):970-975.

[11] ZIETKIEWICZ E,RAFALSKI A,and LABUDA D.Genome fingerprintingby simple sequence repeat(SSR)-anchored polymerase chain reaction, amplification,Genomics,1994,20(2):176-183.

[12] REDDY M P,SARLA N,SIDDIQ E A.Inter simple sequence repeat (ISSR)polymorphism and its application in plant breeding[J].Euphytica,2002,128(1):9-17.

[13] 姚明哲,黄海涛,余继忠,等. ISSR在茶树品种分子鉴别和亲缘关系研究中的适用性分析[J].茶叶科学,2005,25(2):153-157.

[14] 林郑和,陈荣冰,陈常颂,等.ISSR分子标记在茶树遗传关系分析中的初步应用[J].茶叶科学,2007,27(1):45-50.

[15] 唐玉海,郭春芳,张木清,等.ISSR标记在茶树品种遗传多态性研究中的应用[J].福建农林大学学报:自然科学版,2007,36(1):51-55.

[16] 黄建安,黄意欢,罗军武,等.茶树基因组DNA的高效提取方法[J].湖南农业大学学报:自然科学版,2003,29(5):402-407.

[17] 詹梓金,李宗恒,徐飚,等.保护铁观音原种保持优异品质——论铁观音品种提纯复壮[J].茶叶科学技术,2006,(2):20.

[18] 詹梓金,林锻炼,陈磊.安溪铁观音品种复壮内涵与防止退化对策[J].福建茶叶,2009,(1):12-13.

[19] 景润春,何予卿,黄青阳,等.水稻野败型细胞质雄性不育恢复基因的ISSR和SSLP标记分析[J].中国农业科学,2000,33(2):1-9.

[20] KAUNDUN S S,ZHYVOLOUP A,PARK Y G.Evaluation of the genetic diversity among elite tea (Camellia sinensis var.sinensis) accessions using RAPD markers[J].Euphytica,2000,115:7-16.

[21] PARK Y G,SHIV S K.Genetic variation on wild tea populations in Korea[M]//Liang Y R,Liu Z S,Park Y G,et al.Proceedings of the International Symposium on Molecular Biology and TeaBreeding.Hangzhou:Zhejiang University,2000:1-18.

[22] WACHIRA F N,WAUGH R,HACKETT C A,et al.Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers[J].Genome,1995,38:201-210.

[23] MIRSHRA R K,SEN-MANDI S.Genetic diversity estimates for Darjeeling tea clones based on amplified fragment length polymorphism markers[J].J Tea Sci,2004,24(2):86-92.

[24] 刘本英,孙雪梅,李友勇,等.基于EST-SSR标记的云南无性系茶树良种遗传多样性分析及指纹图谱构建[J].茶叶科学,2012,32(3):261-268.

[25] 杨亚军,梁月荣.中国无性系茶树品种志[M].上海:上海科学技术出版社,2014:111,146.

Genetic Diversity of Tieguanyin and Huangdan Half-sib Tea Cultivars Determined by Using ISSR Markers

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	XU Hui-long, WANG Ying-jun, CHEN Ming, FAN Xiao-fang, ZHUO Fei-ying, WEI Yi-cong, FAN Shi-ming. Identification and Genetic Diversity of Polygonatum cyrtonema Hua and P.filipes Merr. Based on ISSR Marker [J]. Fujian Journal of Agricultural Sciences, 2017, 32(6): 619-624.
[2]	WANG Jian-sheng, HOU Gui-ling, CHENG Li-ping, XIE Yong-feng. Genetic Diversity of Nineteen Alfalfa (Medicago L.)Germplasms Analyzed Using ISSR Markers [J]. Fujian Journal of Agricultural Sciences, 2016, 31(7): 708-713.
[3]	ZHONG Huai-qin, LIN Rong-yan, HUANG Min-ling, LIN Bing, Chen Nan-chuan. SRAP-marker Based Genetic Diversity of Germplasms of Cymbidium Hybrids [J]. Fujian Journal of Agricultural Sciences, 2016, 31(11): 1193-1197.
[4]	KANG Zhi-ming, ZHENG Kai-bin, XU Xiao-yu, Li Ai-ping. Genetic Diversity of Faba Bean Varieties in Agronomic and Quality Traits [J]. Fujian Journal of Agricultural Sciences, 2015, 30(3): 249-252.
[5]	LI Ming-song, WU Li, LÜ Mei-qing, KANG Rong-rong, WANG Jin-xian, ZENG Hong-ying. Genetic Diversity of Spring Soybean Landraces in Fujian [J]. Fujian Journal of Agricultural Sciences, 2014, 29(6): 524-529.
[6]	ZHENG Shan, ZHANG Li-jie, XIE Li-xue, ZHANG Xiao-yan, LI Tao. DNA Fingerprinting by ISSR Markers for 17 Blueberries [J]. Fujian Journal of Agricultural Sciences, 2014, 29(12): 1198-1201.
[7]	LI Qi-xiang, HUA Shu-mei, LEI Fu-gui, CHEN Zhi-hua, HE Pei-zhen, CAO Yi-yang, ZHOU Jian-jin. Comparison of ISSR and SRAP Markers on the Genetic Diversity Analysis of Dioscoreae [J]. Fujian Journal of Agricultural Sciences, 2013, 28(9): 876-883.
[8]	XIAO Dong-lai, CHEN Yu-hang, YANG Jing, HUANG Xiao-jing. Phylogenetic Diversity of Russula griseocarnosa from Fujian [J]. Fujian Journal of Agricultural Sciences, 2013, 28(9): 902-905.
[9]	LI Qi-xiang, ZHANG Xiao-zhong, TU Qian-cheng, LEI Fu-gui, JI Ping, CHEN You-yu. Analysis of Genetic Diversity Based on SSR Markers on Silage Maize Inbred Lines [J]. Fujian Journal of Agricultural Sciences, 2013, 28(4): 320-323.
[10]	YANG Wang-xing, XU Xu-ming, QI Jian-min, ZHUO Wei, ZHANG Shou-gang, MA Bin-lin, WEI Xin-yu, ZOU Wen-guang. Genetic Diversity of Parents and Derived Lines Based on Phenotypic Characters and SSR Cluster Analysis [J]. Fujian Journal of Agricultural Sciences, 2013, 28(3): 211-216.
[11]	HUA Xiu-feng, ZHONG Feng-lin, LIN Yi-zhang, CHEN Wen-hui, CHEN Xian, ZHU Hai-sheng. Genetic Diversity Analysis of Strawberry Accession with ISSR [J]. Fujian Journal of Agricultural Sciences, 2013, 28(3): 232-236.
[12]	LI Jian-lin, LI Hong-xia, TANG Yong-kai, YU Ju-hua, DONG Zai-jie . Genetic Diversity Analysis of Six Populations of Common Carp by Microsatellite Markers [J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 936-940.
[13]	LIN Hui, LI Yong-ping, ZHU Hai-sheng, WEN Qing-fang. Analysis of Genetic Diversity Based on Morphological Markers Among Cauliflower Cultivars [J]. Fujian Journal of Agricultural Sciences, 2012, (5): 491-497.
[14]	ZHENG Yu, ZHAO Jian-wei, HE Yu-xian, HUANG jian. Genetic Diversity of Bemisia tabaci(Gennadius) Natural Populations in Fujian Province Based on RAPD and ISSR Marker [J]. Fujian Journal of Agricultural Sciences, 2012, (5): 518-522.
[15]	WANG Zi-xuan, LIU Bo, LIN Ying-zhi, LIU Guo-hong. Collection, Identification and Phylogenetic Diversity of Bacillus species in Soil Samples from Xinjiang [J]. Fujian Journal of Agricultural Sciences, 2012, (2): 187-195.