Fujian Journal of Agricultural Sciences ›› 2016, Vol. 31 ›› Issue (11): 1193-1197.doi: 10.19303/j.issn.1008-0384.2016.11.010

• Horticultural Science • Previous Articles     Next Articles

SRAP-marker Based Genetic Diversity of Germplasms of Cymbidium Hybrids

ZHONG Huai-qin1,2,3, LIN Rong-yan1,2,3, HUANG Min-ling1,2,3, LIN Bing1,2,3, Chen Nan-chuan4   

  1. 1. Institute of Crop Sciences, Fujian Academy of Agricultural Science, Fuzhou, Fujian 350013, China;
    2. Flowers Research Center, Fujian Academy of Agricultural Science, Fuzhou, Fujian 350013, China;
    3. Fujian Engineering Research Center for Characteristic Floriculture, Fuzhou, Fujian 350013, China;
    4. Fujian Bainong Ecological Technology Limited Company, Zhaoan, Fujian 363500, China
  • Received:2016-05-26 Revised:2016-07-30 Online:2016-11-28 Published:2016-11-28

Abstract: SRAP was applied for the determination of the genetic diversity of 24 hybrid cultivars of Cymbidium. Eleven polymorphic primer combinations were screened to result in 119 amplified DNA bands. Of the amplified DNA, 102 were polymorphic, at a ratio of 86.1%. The average number of polymorphic loci amplified by each primer was 9.3. The genetic similarity among the 24 germplasms ranged from 0.43 to 0.98, averaging 0.67. The UPGMA cluster analysis classified the germplasms with a similarity coefficient of 0.69 into 5 groups clearly showing the genetic diversity and relationship among the hybrids.

Key words: Cymbidium hybrid, SRAP, genetic diversity, cluster analysis

CLC Number: 

  • S68