黄曲条跳甲高致病力绿僵菌的筛选及培养特性研究

doi:10.19303/j.issn.1008-0384.2017.02.017

Abstract

Abstract: This study attempted to find a microorganism that could be effectively used for the bio-control of the major pestinsect,Phyllotreta striolata, on vegetables.Metarhizium anisopliae is known as apathogenic fungus that infects the pest. This experimentation evaluated the virulence of 8 M. anisopliae strains against P. striolata. One strainthat exhibited a high activity was Ma6. The bioassay showed a LT₅₀ on adult P. striolata inoculated with 8.0×10⁸ Ma6 spores·mL^-1 at 25℃ to be 4.09 d, and a 100% cumulative mortality rateat 25℃ for 7 d. It was, thus, concluded that Ma6 could be a potential candidate for the pest control. Subsequently, studies showed that the optimum medium for the hyphal growth and sporulation of Ma6 was PDA at 25℃ and pH 7.0 with 6 d in the dark followed by 8 d of sun exposure.

Key words: bio-control, pathogenicity, cultural conditions, sporulation condition

CLC Number:

S435

HE Yue-chao, CHEN Jiang, SHI Meng-zhu, LI Jian-yu, WANG Ting, FU Jian-wei, WU Mei-xiang. Screening and Culture of a Strain of Metarhizium Highly Pathogenic to Phyllotreta striolata[J].Fujian Journal of Agricultural Sciences, 2017, 32(2): 189-194.

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Screening and Culture of a Strain of Metarhizium Highly Pathogenic to Phyllotreta striolata

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