检测猪源牛病毒性腹泻病毒荧光RT-PCR方法的建立与监测分析

doi:10.19303/j.issn.1008-0384.2017.08.004

Fujian Journal of Agricultural Sciences ›› 2017, Vol. 32 ›› Issue (8): 828-832.doi: 10.19303/j.issn.1008-0384.2017.08.004

• Animal Science • Previous Articles Next Articles

Establishment and Monitoring Analysis of Fluorescence RT-PCR for Detection of Bovine Viral Diarrhea Virus in Swine

BAI Quan-yang¹, XU Lei², FU Guang-hua³, ZENG Liang-ming⁴, CHENG Long-fei³, HUANG Yu³

1. Inspection and Quarantine Technology Center of Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou, Fujian 350001, China;
2. Fujian Agricultural Vocational Technical College, Fuzhou, Fujian 350119, China;
3. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
4. Fuzhou Da Bei Nong Biotech Company Limited, Fuzhou, Fujian 350014, China

Received:2017-06-02 Revised:2017-07-14 Online:2017-08-28 Published:2017-08-28

Abstract

Abstract: The study was aimed at developing a quantitative real-time fluorescence reverse transcriptase PCR(qRT-PCR) assay according to the conserved gene sequence of BVDV 5'-UTR for detection of bovine viral diarrhea virus(BVDV) obtained from 359 slaughtered swine serum samples of slaughterhouses in Fuzhou Prefecture. The results showed that the BVDV positive rate of slaughtered swines was 14.5%(52 positive samples out of 359 ones). Sequence analysis on 5'-UTR fragments of 13 BVDV positive samples showed that 5'-UTR fragments of all tested samples were closely related to VEDEVAC evolution lineages, all of which were genotype I. It was indicated that the specific qRT-PCR method established based on BVDV was suitable for detection of BVDV in swines. And the BVDV infection was common in slaughtered swines of Fuzhou Prefecture.

Key words: BVDV in swine, fluorescence RT-PCR, slaughtered swine, pathogenic detection

CLC Number:

S852.65

BAI Quan-yang, XU Lei, FU Guang-hua, ZENG Liang-ming, CHENG Long-fei, HUANG Yu. Establishment and Monitoring Analysis of Fluorescence RT-PCR for Detection of Bovine Viral Diarrhea Virus in Swine[J].Fujian Journal of Agricultural Sciences, 2017, 32(8): 828-832.

References

[1] FAUQUET C. Virus taxonomy:classification and nomenclature of viruses:eighth report of the International Committee on the Taxonomy of Viruses[M]. Academic Press, 2005.

[2] FERNELIUS A L, AMTOWER W C, LAMBERT G, et al. Bovine Viral Diarrhea Virus in swine:characteristics of virus recovered from naturally and experimentally infected swine[J]. Can J Comp Med, 1973, 37(1):13-20.

[3] 朱礼倩, 周艳君, 于海,等. 牛病毒性腹泻在中国的流行现状分析[J]. 中国动物传染病学报, 2011, 19(5):83-86.

[4] 徐磊,曾亮明,王玉玲,等.福建省猪群感染牛病毒性腹泻病毒的病原学检测与分析[J].畜牧兽医学报,2014,45(12):2006-2012.

[5] 曾亮明,徐磊,王玉玲,等.猪血清中牛病毒性腹泻病毒抗体ELISA检测方法的建立与应用[J].中国兽医科学,2014,44(09):944-950.

[6] 吴文辉.猪群BVDV感染状况调查及成因初步分析[D].上海:上海交通大学,2011.

[7] 邓宇,张荣,丛雁方,等.猪源牛病毒性腹泻病毒实时荧光定量PCR检测方法的建立[J].畜牧兽医学报,2011,42(7):1046-1050.

[8] 王新平,涂长春,李红卫,等.从可疑猪瘟病料中检出牛病毒性腹泻粘膜病病毒[J].中国兽医学报,1996,16(4):341-345.

[9] 孙泉云,张苏华,沈悦,等.奶牛和猪血清中牛病毒性腹泻-粘膜病抗体的检测[J].畜牧与兽医,2004,36(2):30-31.

[10] 宋永峰,张志,张燕霞,等.猪源牛病毒性腹泻病毒的流行初探[J].中国动物检疫,2008,25(7):25-27.

[11] TERPSTRA C, WENSVOORT G. Natural infections of pigs with bovine viral diarrhea virus associated with signs resembling swine fever[J]. Res Vet Sci, 1988, 45(2):137-142.

[12] TOPLAK I, SANDVIK T, BARLI-MAGANJA D, et al. Genetic typing of bovine viral diarrhoea virus:most Slovenian isolates are of genotypes 1d and 1f[J]. Veterinary Microbiology, 2004, 99(3-4):175.

[13] CAI D, SONG Q, WANG J, et al. Genomic characterization of three bovine viral diarrhea virus isolates from cattle.[J]. Archives of Virology, 2016, 161(12):1-4.

Establishment and Monitoring Analysis of Fluorescence RT-PCR for Detection of Bovine Viral Diarrhea Virus in Swine

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 2

Metrics

Comments

Recommended 0

[1]	FANG Gui-you, WANG Long-bai, ZHUANG Xiang-sheng, SHAO Liang-ping, ZHOU Lun-jiang, CHE Yong-liang, WEI Hong, CHEN Ru-jing. The Research of Highly Pathogenic PRRSV Distribution in Challenged Pigs [J]. Fujian Journal of Agricultural Sciences, 2011, (3): 329-334.
[2]	BAI Quan-yang, SUN Ying-jie, ZHENG Teng, ZHANG Bo-qiang, ZHAO Xing-cun, WANG Wu-jun, ZHANG Zhi-deng, ZHANG Ti-yin. Indirect immunity fluorescence assay on H9 subtype avian influenza virus and comparison with fluorescence RT-PCR method [J]. Fujian Journal of Agricultural Sciences, 2008, (2): 146-148.