新-法二联灭活疫苗免疫不同日龄商品蛋鸡的效果

doi:10.19303/j.issn.1008-0384.2017.09.002

Abstract

Abstract: Immunological effect of combined inoculation of the inactivated vaccines for Newcastleand the infectious bursal diseases (ND-IBD) on egg-laying chickens was investigated. For the study, 360 one-day-old commercial chicks were randomly divided into 3 groups. The chicks in one group were immunized immediately with the two vaccines simultaneously, another when they were 7-day-old, and the third group injected with saline solution as control.Levels of ND-IBD antibodies in the serum ofthese chicks were determined, spleen biopsies for disease detection executed, and qPCR on mRNA expressions of CD4⁺, CD8⁺, IL-6 and IFN-β performed every 7 days during the period of 21 to 56 d. The results showed that all treated one-or 7-day-old birds had higher antibody levels than control, and could fence off IBD virus infection. The 7-day-old group showed greater immunity against the diseases, and significantly increased mRNA expressions on CD4⁺, CD8⁺, IL-6 and IFN-β over control. It was, therefore, concluded that,by combining vaccinations against ND-IBD on 7-day-old egg-laying chicks,a desirable immunological effect on the chickens could be achieved.

Key words: newcastle disease, infectious bursal disease, inactivated vaccine, antibody detection

CLC Number:

S858.31

XING Dong, LIU Meng-xi, YUAN Xiao-qin, LI Chun-yan, CHEN Shi-yi, JIANG Xing-hua, ZHAO Hua-e, BAO Song-ying, WANG Quan-xi. Immunological Effect on Egg-laying Chickens by Combining Vaccinations Against Newcastle and Infectious Bursal Diseases[J].Fujian Journal of Agricultural Sciences, 2017, 32(9): 926-931.

References

[1] MULLER H, ISLAM M R, RAUE R. Research on infectious bursal disease the past, the present and the future[J]. Vet Microbiol, 2003, 97(1/2):153-165.

[2] 王志全, 丁鹏. 新城疫研究进展及综合防治[J]. 中国畜禽种业, 2014,10(2):136-137.

[3] 李成洪, 王建华, 段霖. 蜂胶提取物对鸡传染性法氏囊病疫苗免疫作用的影响[J]. 黑龙江畜牧兽医, 2017,(8):151-154.

[4] 梅永杰, 王永山, 欧阳伟, 等. 华东地区传染性法氏囊病病毒流行株的分离及其生物学特性分析[J]. 中国兽医科学, 2016,46(5):550-557.

[5] 李潭清,宋勤叶,杨润德,等.鸡传染性法氏囊病的研究进展[J].安徽农业科学,2008,36(35):15481-15484.

[6] 江兴华, 林晓荣, 星东, 等. 鸡新城疫、传染性法氏囊病二联灭活疫苗免疫效果研究[J]. 黑龙江畜牧兽医, 2017,(3):108-110.

[7] 于晓雪. 鸡CD4⁺CD25⁺细胞在传染性法氏囊病和免疫耐受诱导过程中的作用[D]. 北京:中国农业大学, 2016.

[8] 闫若潜, 谢彩华, 吴志明, 等. 重组鸡白细胞介素-6(rChIL-6)对不同种类疫苗免疫增强作用研究[C]//中国畜牧兽医学会禽病学分会第十五次学术研讨会论文集. 2010.

[9] 张忠民. IL-6与NF-κB在人脑胶质瘤中的表达及相关性研究[D].佳木斯:佳木斯大学, 2015.

[10] 陈建忠, 朱海红, 陈智,等. IFN-α/β信号传导研究进展[J]. 国际流行病学传染病学杂志, 2003, 30(5):266-268.

[11] 孙恒. 鸡新城疫的疫苗接种方式与免疫程序[J]. 养殖技术顾问, 2012,(3):206-206.

[12] 赵占民, 赵来兵, 李全福, 等. 母源抗体对鸡新城疫疫苗免疫的影响[J]. 中国兽医杂志, 2004, 40(3):6-8.

[13] 章振华, 李林, 景小冬, 等. 鸡传染性法氏囊病免疫复合物疫苗对1日龄中等母源抗体水平雏鸡免疫效果的研究[J]. 中国畜牧兽医, 2015, 42(12):3338-3343.

[14] 简子健, 曹竹亭, 马素贞,等. 单稀释法间接ELISA和终点法中和试验检测犊牛轮状病毒疫苗抗体效价比较[J]. 新疆农业科学, 2008, 45(4):733-737.

[15] 侯继波, 何家惠, 惠艳华,等. 鸡传染性支气管炎病毒血凝抑制试验研究[J]. 中国家禽, 2007, 29(1):20-22.

[16] 黄劲, 邓永荣, 戴荣旺,等. 中药制剂对禽流感病毒H9N2、新城疫病毒、传染性支气管炎病毒和鸡毒支原体的攻毒保护试验[J]. 动物医学进展, 2005, 26(12):83-86.

[17] 孙德君,丁国杰,藏玉婷,等.鸡传染性法氏囊病特点、诊断及国内外灭活疫苗免疫效力比较[J]. 科技与实践, 2012,50(20):76-78.

[18] 王秀荣, 王笑梅, 吴东来,等. 鸡贫血病灭活疫苗免疫后雏鸡免疫状况研究[J]. 中国预防兽医学报, 2000,23(S1):63-65.

[19] 季卫国, 方占海, 周月琴. 鸡传染性法氏囊病的综合防控[J]. 养殖与饲料, 2015,(8):52-53.

[20] RAUTENSCHLEINS,YEH H Y,SHARMA J M. The role of T cells in protection by an inactivated infectious bursal disease virus vaccine[J].Veterinary immunology and immunopathology, 2002, 89(3/4):159-167.

[21] 刘蕊娜,邹年莉,王红宁,等.鸡IL-6真核表达载体的构建及其对新城疫LaSota疫苗的免疫增强作用研究[J].畜牧兽医学报, 2009, 40(1):93-97.

[22] BLOCK H, MEYE R-BLOCK K,REBESKID,et al. A field study on the significance of vaccination against infectious bursal disease virus (IBDV) at the optimal time point in broiler flocks with maternally derived IBDV antibodies[J].Avianpathology, 2007,36(5):401-409.

[23] 梁华丽, 华炯钢, 徐辉,等. 几种鸡新城疫油佐剂灭活疫苗免疫效果的比较[J]. 浙江农业学报, 2007, 19(6):418-422.

Immunological Effect on Egg-laying Chickens by Combining Vaccinations Against Newcastle and Infectious Bursal Diseases

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