微波辅助提取黄秋葵黄酮类化合物及抗氧化活性

doi:10.19303/j.issn.1008-0384.2018.06.019

Abstract

Abstract: A microwave-assisted processes to extract flavonoids from Abelmoschus esculentus was optimized by a single factor and orthogonal experiment. By using 70% ethanol as the solvent, A. esculentus in a solid-liquid ratio of 1:40 (g·mL^-1)was extracted at 70℃ for 40 minutes. The yield of flavonoids was 2.78% with a relative standard deviation of 0.635%. The resulting extract was tested for its antioxidant activity by means of DPPH and ABTS radical scavenging capabilities to show IC₅₀at 1.21 mg·mL^-1 and 1.06 mg·mL^-1, respectively. It appeared that the current method could render a high yield on flavonoids that showed a substantial antioxidative activity.

Key words: microwave, Abelmoschus esculeutus, flavonoids, extraction, antioxidant activity

CLC Number:

S662

DENG Hao, YIN Qing-chun, ZHANG Rong-hu, FENG Jian-cheng, YU Huan-huan. Process and Antioxidant Activity of Flavonoid Extract from Abelmoschus esculentus[J].Fujian Journal of Agricultural Sciences, 2018, 33(6): 649-654.

References

[1] 单承莺, 马世宏, 张卫明. 保健蔬菜黄秋葵的应用价值与前景[J]. 中国野生植物资源, 2012, 31(2):68-71.
[2] 许俊齐. 黄秋葵净菜加工及MAP保鲜关键工艺研究[D]. 福州:福建农林大学, 2013.
[3] 薛志忠, 刘思雨,杨雅华. 黄秋葵的应用价值与开发利用研究进展[J]. 保鲜与加工, 2013, 13(2):58-60.
[4] CHENG J, CHEN X, SHENG Z, et al. Antioxidant-capacity-based models for the prediction of acrylamide reduction by flavonoids[J].Food Chemistry, 2015, 168:90-99.
[5] 白阳, 孙正旺, 刘春莹,等. 甘草总黄酮的制备及其抗皮肤老化功能[J]. 大连工业大学学报, 2015,(5):317-319.
[6] 吴晓光, 李玲, 苗光新,等. 山楂叶总黄酮对血管性痴呆大鼠学习记忆的干预作用及机制[J]. 中国老年学杂志, 2015,(14):3819-3822.
[7] 王博, 黄翊鹏, 曲静然. 黄秋葵中黄酮的提取工艺研究[J]. 齐鲁工业大学学报, 2015,29(3):61-64.
[8] 张泽生, 董晓朦, 王田心,等. 黄秋葵中多酚和黄酮提取工艺的研究[J]. 中国食品添加剂, 2017,(1):91-99.
[9] 陈宝宏, 马震雷, 孙莹莹. 黄秋葵花中黄酮类物质的提取及抗氧化性分析[J]. 江苏调味副食品, 2017,(3):20-23.
[10] SUN T, HO C T. Antioxidant activities of buckwheat extracts[J].Food Chemistry, 2005, 90(4):743-749.
[11] DUDONNÉ S, VITRAC X, COUTIẼRE P, et al. Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays.[J]. Journal of Agricultural & Food Chemistry, 2009, 57(5):1768-74.
[12] FANG Z, ZHANG Y, YUAN L, et al. Phenolic compounds and antioxidant capacities of bayberry juices[J]. Food Chemistry, 2009, 113(4):884-888.
[13] 王广慧. 微波辅助高压法提取金针菇黄酮条件的优化[J]. 食品研究与开发, 2017, 38(8):46-49.
[14] 陈婷, 徐文斌, 洪怡蓝,等. 响应面法优化甜叶菊残渣中总黄酮提取工艺及抗氧化活性[J]. 食品科学, 2016, 37(18):52-57.
[15] 清源. 苦荞茶中黄酮提取工艺优化及不同品牌苦荞茶黄酮含量比较[J]. 南方农业, 2016, 10(3):1-5.
[16] 赵晨晨, 承伟, 王立冬. 微波辅助提取络石藤总黄酮的工艺研究[J]. 中草药, 2012, 43(4):718-720.
[17] 王秋实, 李凤林. 玉米须黄酮利用肝癌细胞降糖实验研究[J]. 吉林农业科技学院学报, 2017(3):10-13.
[18] 徐天姿, 单雪峰, 孙炜,等. 黄秋葵黄酮抗小鼠运动性疲劳的作用及其机理研究[J]. 中华中医药学刊, 2014,32(4):880-882.
[19] 李筱玲, 邓寒霜. 黄酮类化合物提取分离方法研究进展[J]. 陕西农业科学, 2015,61(1):77-79.

Process and Antioxidant Activity of Flavonoid Extract from Abelmoschus esculentus

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	CHEN Li-lin, LIN Chang-jin, LAI Zhi-li, SHI Fan, WENG Xi-fang, CAI Jing-long. Insecticidal Efficacy of Microwave on Empoasca onukii (Hemiptera, Cicadellidae) [J]. Fujian Journal of Agricultural Sciences, 2018, 33(12): 1301-1306.
[2]	HUANG Qiong, HE Yan-ping. Optimization of Ultrasound/Microwave-assisted Extraction of Polysaccharides from Hibiscus sabdariffa L. by Box-Behnken Response Surface Methodology [J]. Fujian Journal of Agricultural Sciences, 2018, 33(12): 1324-1329.
[3]	ZOU Yi-hui, LIU Chang-yong, LIN Ze-yan, LI Zhen. Transcriptome Sequencing and Flavonoid Biosynthesis-related Genes of Scutellaria barbata D. Don [J]. Fujian Journal of Agricultural Sciences, 2018, 33(12): 1242-1250.
[4]	ZHANG Shuai, ZHENG Kai-bin, DING Zi-qiu, ZHANG Shao-ping, QIU Shan-lian. Development of Low-sugar and Antioxidant Beverage with Purslane Portulaca oleracea L. [J]. Fujian Journal of Agricultural Sciences, 2018, 33(11): 1206-1211.
[5]	LIN Zhi-luan, JIN Xiao-huai, ZHANG Chuan-hai, LI Bao-yin, HUA Wei-ping. Content and Bioactivity of Flavonoids Extracted from Mucunae sempervirens and Extraction Process Optimization [J]. Fujian Journal of Agricultural Sciences, 2018, 33(11): 1212-1218.
[6]	LI Cheng-xun, XU Xiao-yu, WU Si-feng, LI Ai-ping. Ultrasound Extraction of Flavonoids from Cymbidium kanran Flowers [J]. Fujian Journal of Agricultural Sciences, 2018, 33(11): 1219-1223.
[7]	HE Shu-lan, RAO Xue-e, YANG You-fen, PAN Da-ren. Macroporous Resin for Purifying Flavonoids from Leaves of Helianthus tuberosus L. [J]. Fujian Journal of Agricultural Sciences, 2017, 32(9): 981-986.
[8]	HE Shu-lan, ZHU Fu-rong, ZHU Hong-yang, RAO Xue-e, LUO Wen-fang. Optimization of Flavonoids Extraction from Leaves of Helianthus tuberosus L. by Response Surface Methodology and Determination of Antimicrobial Activity of Resulting Extract [J]. Fujian Journal of Agricultural Sciences, 2017, 32(8): 897-904.
[9]	YANG Jun-guo, WANG Li-li, SONG Zhen-shuo, CHEN Jian, CHEN Lin. Compositions and Antioxidant Activities of Oolong Tea Extracts Made by Two Processing Methods [J]. Fujian Journal of Agricultural Sciences, 2017, 32(8): 891-896.
[10]	LI An, LIU Yin, ZHANG Ze-wang, MENG Yu-shi, WU De-zhi. The Study on the Microwave-Assisted Extraction on Essential Oil of Blumea balsamifera (L.) DCand Antibacterial Activity [J]. Fujian Journal of Agricultural Sciences, 2017, 32(7): 751-755.
[11]	ZHAO Yun-qing, HUANG Ying-zhen, CHEN Jing-ying, LIU Bao-cai, HUANG Rui-ping. Quality and Functional Ingredients of Anoectochilus roxburghii (Wall.) Lindl. Dried at Various Dehydration Temperatures [J]. Fujian Journal of Agricultural Sciences, 2017, 32(7): 807-809.
[12]	CHEN Xiao-ning, ZHENG Li-zhu, WEI Qi, LIN Cheng, CHEN Bing-zhi, XIAO Shu-xia, JIANG Yu-ji. Optimization of Ethanol Extraction of Antibacterial Substances from Dictyophora indusial Fisscher [J]. Fujian Journal of Agricultural Sciences, 2017, 32(6): 645-651.
[13]	LIN Zhi-luan, XIE Dan-hua, YAN Li-ling, JIN Xiao-huai, ZHANG Chuan-hai, LI Bao-yin. Optimization of Flavonoid Extraction from Lysimachia patungensis [J]. Fujian Journal of Agricultural Sciences, 2017, 32(6): 660-664.
[14]	YANG Shu-yun. Optimization of Polysaccharide Extraction from Mycelia of Selenium-rich Agaricus blazei [J]. Fujian Journal of Agricultural Sciences, 2017, 32(5): 531-535.
[15]	GAO Xiang-feng, WANG Rang-jian, LIU Feng-jing, LI Hui-ling. Hydroponic Culturein Sample Preparation of Tea Shoots for RNA Sequencing [J]. Fujian Journal of Agricultural Sciences, 2017, 32(2): 155-160.