紫背天葵及其近缘种白子菜花青素合成相关基因分析

doi:10.19303/j.issn.1008-0384.2019.05.003

Abstract

Abstract: [Objective] The core enzymes in anthocyanin anabolism were investigated,to provid a reference for further studies on the anthocyanin metabolism of G. bicolor and similar plants.[Method]Transcriptome of Gynura bicolor and G. divaricata were sequenced using the Illumina HiSeq 2 500 platform for the study.[Result] The Denovo mapping on 8 databases showed that 43 369 out of 80 798 unigenes annotated differences between the two wild vegetables. Among them, 104 unigenes were identified to relate to the anthocyanin anabolism based on two databases on the non-redundant and Swiss-Prot proteins. They included 10 chalcone synthases, 5 chalcone isomerases, 36 flavanone 3-hydroxylases, 19 dihydroflavonols, 9 anthocyanidin synthases, and 25 flavonoid 3-O-glucosyl transferases. According to the average FPKMs, 7 of the 15 differentially expressed genes (i.e., 1, 1, 3, 4, 2, and 4 genes from each of the 6 enzyme categories) between the 2 wild vegetables were found to be up-regulated, and 8 down-regulated.[Conclusion] There were 15 enzymes in the 104 unigenes appeared to closely associate with the anthocyanin synthesis in G. bicolor and G. divaricata.

Key words: Gynura bicolor, Gynura divaricate, transcriptome sequencing, anthocyanin, metabolic enzyme

CLC Number:

S661.2

ZHANG Shao-ping, QIU Shan-lian, ZHANG Shuai, HONG Jia-min, LIN Bao-mei, ZHENG Kai-bin. Transcriptome Analysis on Anthocyanin Anabolism Genes in Gynura bicolor and Gynura divaricata[J].Fujian Journal of Agricultural Sciences, 2019, 34(5): 516-524.

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Transcriptome Analysis on Anthocyanin Anabolism Genes in Gynura bicolor and Gynura divaricata

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