晾晒烟种质资源ISSR指纹图谱构建及遗传变异分析

doi:10.19303/j.issn.1008-0384.2019.05.004

Abstract

Abstract: [Objective]This study was aimed to accurately reveal the genetic relationship among sun-cured tobacco germplasms, and improve the utilization and breeding efficiency of sun-cured tobacco resources.[Method]Genetic diversity of 36 sun-cured tobacco germplasms was analyzed using ISSR.[Result]From the samples, 113 DNA bands were amplified with 7 polymorphic primers selected from 100 ISSR primers. Among them, 91 bands (80.5%) were polymorphic. The average effective number of alleles (Ne), Nei's gene diversity index (H),and Shannon's information index (I) of the ISSR markers were 1.64, 0.37, and 0.55, respectively, indicating a high detection efficacy. The UPGMA analysis showed a low diversity among the germplasms with the genetic similarity coefficients (GS) ranging from 0.74 to 0.91. With the new method, the germplasms were clustered into 5 groups at the level of 0.78.The digital fingerprints effectively distinguish the germplasms.[Conclusion]The ISSR markers chosen for the DNA fingerprints of 36 sun-cured tobacco germplasms were appropriate for the otherwise difficult task of differentiating and identifying those genetically closely related varieties.

Key words: sun-cured tobacco, ISSR, fingerprint maps, genetic diversity

CLC Number:

S323

QIN Jian-feng, HE Jiang, CHEN Tao, QIN Xu, CUI Ming-yong, HUANG Xian-ya, MENG Yao, LIANG Chun. ISSR Fingerprints and Genetic Variations of Sun-Cured Tobacco Germplasms[J].Fujian Journal of Agricultural Sciences, 2019, 34(5): 525-533.

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ISSR Fingerprints and Genetic Variations of Sun-Cured Tobacco Germplasms

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