Fujian Journal of Agricultural Sciences ›› 2007, Vol. ›› Issue (4): 410-413.

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Extracting condition and the activity admeasurement for pectinesterase in achenes of Ficus awkeotsang Makino

WU Wen-shan1,2, XIE Qing-qing3, ZHENG Cui-fang2, ZHU Xiao-dong2, LIN Wei2, HUANG Mei-li2   

  1. 1. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
    2. College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350007, China;
    3. College of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China
  • Received:2007-01-16 Revised:2007-09-18 Online:2007-12-15 Published:2007-12-15

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Abstract: A method of extraction and assay for the pectinesterase were investigated and proposed as follows: the best concentration of NaCl in extracting mixtrure is 5%,the best extracting temperature is 30℃,the best extracting time is 24 hours,the best conservating time is 24 hours,the best concentration of NaCl in mixture is 0.6% to 1.0%,and maintain the pH on 7.0.Between 30-50℃,the pectinesterase in achenes of Ficus awkeotsang has a good thermal stability.

Key words: Ficus awkeotsang Makino achenes, pectinesterase activity, extracting condition

CLC Number: 

  • TS201.25
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