Abstract: Anti-fungal activities of the extracts from Sargassum fusiforme were tested by means of hypha growth rate and spore germination.The results indicated that the R_f0.47 at 10 g•kg^-1,1 g•kg^-1 and R_f0.47 at 100 g•kg^-1,1 g•kg^-1 of the ethyl acetate phase on TLC from the S.fusiforme soaked in ethanol significantly inhibited Penicillium cyclopium growth.The inhibition rates of those components were 35%,50%,85% and 25%,respectively.The R_f0.30 at 100 g•kg^-1 of the same extract significantly inhibited Trichoderma viride and Aspergillus niger V.Tiegh growth with inhibition rates of 75.67% and 83.46%,respectively.The correlation analysis showed that concentrations of the 3 treatment groups significantly correlated to the inhibitory effect.Two-factors analysis of variance showed that the separation and concentration affected significantly on T.viride,P.cyclopium,A.niger V.Tiegh mycelial growth inhibition.In different concentrations,R_f0.47,R_f0.81 of Sargassum fusiforme soaked with ethanol,R_f0.00 of Sargassum fusiforme soaked with ether and ethanol ethyl acetate phase and ethyl acetate phase of Sargassum fusiforme ether,R_f0.30 of Sargassum fusiforme soaked with ether have the extremely significant inhitition on the germination of spores of Aspergillus niger V.Tiegh.

Key words: Sargassum fusiforme, anti-microbial activity, thin layer chromatography(TLC), growth rate method, spore germination method