福州地区甘蔗宿根矮化病致病菌Leifsonia xyli subsp. Xyli PCR检测

Abstract

Abstract: A pair of specific primers were designed to amplify the internal transcript space region of the 16S-23S ribosomal DNA of Leifsonia xyli subsp. xyli.(Lxx),pathogen that causes the ratoon stunting disease(RSD).As a result,a PCR amplification system was established.Four out 5 cultivars planted in the Sugarcane Germplasm Resources Center of the Sugarcane Institute at Fujian Agriculture and Forestry University were infected by the Lxx.The PCR products of the infected plants and a positive control were sequenced and analyzed.The sequences were found to be identical.When.compared the NCBI data,they were 100% homologous on the same genome region of DNA with Lxx of Brazil and Australia.On the other hand,there were a mismatched and an insertion base pair in the Lxx as.compared with the strain from Louisiana,USA.

Key words: RSD, sugarcane, PCR detection

CLC Number:

S435.661

QUE You-xiong, XU Jing-sheng, XU Li-ping, GAO San-ji, CHEN Ru-kai. PCR detection for Leifsonia xyli subsp. Xyli,pathogen of the sugarcane ratoon stunting disease[J].Fujian Journal of Agricultural Sciences, 2008, (4): 364-367.

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PCR detection for Leifsonia xyli subsp. Xyli,pathogen of the sugarcane ratoon stunting disease

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