云引稻瘟病抗性基因型的初步分析及其近等基因系构建

Fujian Journal of Agricultural Sciences ›› 2012, Vol. ›› Issue (1): 1-6.

Preliminary Genotype Analysis of Broad-spectrum Rice Blast Resistance Variety Yunyin and its Near-isogenic Lines Construction

ZHU Yong-sheng^1,2,3,4, CAI Qiu-hua^1,2,3,4, XUE Wei-min^1,2,3,4, XIE Hong-guang^1,2,3,4, WU Fang-xi^1,2,3,4, ZHANG Jian-fu^1,2,3,4, XIE Hua-an^1,2,3,4

1. Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou,Fujian 350019, China;
2. Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture, P.R China/Fuzhou branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou,Fujian 350003, China;
3. Incubator of National key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology, P.R.China, Fuzhou, Fujian 350003, China;
4. National Rice Engineering Laboratory of China, Fuzhou, Fujian 350003, China

Received:2011-11-22 Revised:2011-12-26 Online:2012-01-30 Published:2012-01-30

Abstract

Abstract: The phenotype of broad-spectrum rice blast resistance variety Yunyin was analyzed and identified by inoculated with rice blast fungi RB01-RB22 of known avirulence genes as pathogen in vitro. The results showed that Yunyin was resistant to all rice blast fungi except RB17 and RB20.Analyzing the widely used 24 resistance genes in production at present, the results showed that Yunyin may contain new blast resistance genes besides these genes, and there were able to be applied for rice breeding and production. In addition, in this study, we constructed near-isogenic lines with LTH as genetic background and contained blast-resistant gene to blast isolate SiChuan 43 by successive backcrossing combining marker-assisted selection with artificial inoculation.These works would be beneficial to the genetics study and application of this blast-resistance gene.

Key words: rice blast, resistance gene, identification, near-isogenic lines

CLC Number:

S 511

ZHU Yong-sheng, CAI Qiu-hua, XUE Wei-min, XIE Hong-guang, WU Fang-xi, ZHANG Jian-fu, XIE Hua-an. Preliminary Genotype Analysis of Broad-spectrum Rice Blast Resistance Variety Yunyin and its Near-isogenic Lines Construction[J].Fujian Journal of Agricultural Sciences, 2012, (1): 1-6.

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Preliminary Genotype Analysis of Broad-spectrum Rice Blast Resistance Variety Yunyin and its Near-isogenic Lines Construction

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