Fujian Journal of Agricultural Sciences ›› 2012, Vol. ›› Issue (2): 124-129.

• Animal Science • Previous Articles     Next Articles

Development and Application of the Nested PCR Assay for Duck Flavivirus

ZHANG Lin1,2, HU Bei-xia1,2, YAN Shi-gan1,2, YANG Shao-hua1,2, XU Chuan-tian1,2, ZHANG Xiu-mei1,2   

  1. 1. Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong 250100, China;
    2. Shandong Key Lab of Animal Disease Control and Breeding, Jinan, Shandong 250100, China
  • Received:2011-04-02 Revised:2011-11-19 Online:2012-02-29 Published:2012-02-29

Abstract: Flavivirus universal primer pairs and nested PCR were developed as an efficient method to detect flavivirus, especially new type flavivirus. Four primers Flav P1-Flav P4 located in conserved region of the NS5 gene were designed after alignment of the flavivirus genome and then nested PCR assay was developed for duck flavivirus. Nested PCR was 1 000 times more sensitive than the conventional PCR and it was only able to amplify the flavivirus with the sensibility of 90 copies·μL-1. Phylogenetic analysis showed that duck flavivirus belongs to mosquito-born flavivirus, NTAV group, similar with Tembusu and BYD virus. The clinical samples detection showed that the universal primers and nested PCR were highly specific and sensitive. In addition, the station of evolution of duck flavivirus was clarified.

Key words: Flavivirus, universal primers, nested PCR, duck, sensitivity

CLC Number: 

  • S 852.65