Fujian Journal of Agricultural Sciences ›› 2007, Vol. ›› Issue (4): 469-470.

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A new method—LiCl for extracting bacterium plasmid DNA

PAN Jian-jing1, ZHANG Jun-cheng2, LU Yi-ping1, ZHANG Hang-ying2, ZHUANG Wei-jian1   

  1. 1. College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
    2. Sanming University, Sanming, Fujian 365000, China
  • Received:2007-02-27 Revised:2007-12-12 Online:2007-12-15 Published:2007-12-15

Abstract: A new method to extract small amount of plasmid DNA with LiCl was developed.The results showed that the quality of plasmid DNA extracted by using this method was basically identical to that of the conventional alkalinelysis.The method satisfied the need for basic molecular biology experiments,and the procedure was simplified shortening the extracting time by 24 min.

Key words: Plasmid DNA, LiCl

CLC Number: 

  • Q-33