福建农业学报 ›› 2009, Vol. ›› Issue (1): 29-34.

• 植物保护 • 上一篇    下一篇

生防菌JK-2对尖孢镰刀菌抑制特性的研究

葛慈斌, 刘波, 蓝江林, 黄素芳, 朱育菁   

  1. 福建省农业科学院农业生物资源研究所, 福建, 福州, 350003
  • 收稿日期:2008-06-11 修回日期:2008-12-09 出版日期:2009-02-15 发布日期:2009-02-15
  • 通讯作者: 刘波(1957-),男,博士,研究员,从事微生物生物技术和微生物防治研究(E-mail: fzliubo@163com)
  • 基金资助:
    国家863计划项目(2006AA10A211);福建省自然科学基金项目(2006J0068);福建省科技计划重点项目(2008N0020);福建省财政专项-福建省农业科学院科技创新团队建设基金(STIF-Y03)

Inhibition activity of biocontrol bacteria,strain JK-2,on Fusarium oxysporum

GE Ci-bin, LIU Bo, LAN Jiang-lin, HUANG Su-fang, ZHU Yu-jing   

  1. Institute of Agrobiological Resources, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China
  • Received:2008-06-11 Revised:2008-12-09 Online:2009-02-15 Published:2009-02-15

摘要: 从福建省永泰、福清、闽侯等6个县(市)采集西瓜、番茄和豇豆等作物的根际土壤,用平板稀释法,分离到芽孢杆菌58株。采用目标病原菌多重菌株平行测定法,测定这58个菌株对6株枯萎病尖孢镰刀菌的抑制作用,筛选得到拮抗菌株3株,其中菌株JK-2(短短芽孢杆菌Brevibacillus brevis)的抑菌效果特别显著。对JK-2的抑菌谱调查结果表明,该菌株对大丽轮枝菌(Verticillium dahliae)、黑白轮枝菌(Verticillium alboatrum)、真菌轮枝霉(Verticillium fungicola)、胶孢炭疽菌(Colletotrichum gloeosporioides Penz.)、桃褐腐丛梗孢(Monilia laxa)和青枯雷尔氏菌(Ralstonia solanacearum)都具有较强的抑制作用。JK-2产生的抗菌物质对热的稳定性较强,对蛋白酶K不敏感;盆栽及田间小区试验结果表明,JK-2对西瓜枯萎病的防效分别可达83.60%和78.96%。

关键词: 尖孢镰刀菌, 拮抗菌, 短短芽孢杆菌, 防效

Abstract: Fifty-eight strains of Bacillus spp.were isolated from the rhizospheric soils around watermelon,tomato and other plants at Yongtai,Fuqing,Minhou and 3 other counties in Fujian.Using multiple target pathogens inhibition test on 6 strains of Fusarium oxysporum Schl.,3 showed high inhibition activities.Among these strains of the biocontrol bacteria,Brevibacillus brevis JK-2,was most effective.Strain JK-2 displayed a broad-spectrum antagonistic activity to pathogenic fungi and bacteria,including Verticillium dahliae,Verticillium albo-atrum,Verticillium fungicola,Colletotrichum gloeosporioides,Monilia laxa and Ralstonia solanacearum.The antimicrobial compounds produced by JK-2 were stable to high temperature(i.e.,100℃)and insensitive to proteinase K.It had control efficacies to watermelon wilt at 83.60% and 78.96% in the field and pot culture tests,respectively.

Key words: Fusarium oxysporum Schl., biocontrol bacterium, Brevibacillus brevis, control efficacy

中图分类号: 

  • S435