福建农业学报 ›› 2010, Vol. ›› Issue (6): 663-666.

• 动物科学 •    下一篇

一种引起种(蛋)鸭产蛋骤降新病毒的分离与初步鉴定

万春和1, 施少华1, 程龙飞1, 陈红梅1, 傅光华1, 张大丙2, 林芳1, 林建生1, 黄瑜1   

  1. 1. 福建省农业科学院畜牧兽医研究所, 福建 福州, 350013;
    2. 中国农业大学动物医学院, 北京, 100193
  • 收稿日期:2010-11-26 修回日期:2010-12-10 出版日期:2010-12-15 发布日期:2010-12-15
  • 通讯作者: 黄瑜(1966- ).男,研究员,主要从事畜禽传染病研究(E-mail:huangyu_815@163.com)

A newly identified Flavivirus Virus causing abrupt egg-laying reduction in ducks

WAN Chun-he1, SHI Shao-hua1, CHENG Long-fei1, CHEN Hong-mei1, FU Guang-hua1, ZHANG Da-bing2, LIN Fang1, LIN Jian-sheng1, HUANG Yu1   

  1. 1. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
    2. College of Veterinary Medicine, China Agriculture University, Beijing 100193, China
  • Received:2010-11-26 Revised:2010-12-10 Online:2010-12-15 Published:2010-12-15

摘要: 自表现产蛋骤降的病死种鸭中无菌采集卵巢以番鸭胚分离到病毒,经对分离的病毒(WR株)进行形态学观察、理化特性测定,发现该病毒有囊膜,直径为30~50nm;对氯仿处理敏感;在pH值为7.2条件下,不凝集鸡、鸭和鹅红细胞。人工感染开产麻鸭能复制出与自然感染病例相同的临床症状和病理变化,并能回收到病毒。应用RT-PCR技术对分离毒进行检测,可扩增到约1kb特异性条带。经测序分析表明,所获得的核苷酸序列第9-777位与坦布苏病毒(TMUV)的同源性最高,为88.7%;遗传进化分析表明WR株病毒与坦布苏病毒、圣路易斯脑炎病毒(SLEV)、以色列火鸡脑膜炎病毒(ITV)、恩塔亚病毒(NTAV)具有相似的祖先,但在系统发生树上处于单独的进化分支。由此推测,WR株病毒不属于现有黄病毒科黄病毒属任何一种,为黄病毒属中的新成员,为国内外首次报道。

关键词: 种(蛋)鸭, 产蛋骤降, 黄病毒, 新种, 分离与鉴定

Abstract: A virus WR strain was isolated from the ducks,which experienced abrupt reduction in egg-laying or died of ovarianhemorrhage.The Muscovy ducks inoculated with the WR strain showed pathological effects similar to that of the field cases.The virus could be recovered from the tissues of the sampled ducks in the wild.The viron of the isolated virus had an enveloping membrane and was 30-50 nm in diameter,as observed under electron microscope.The virus WR strain was sensitive to chloroform,and had no haemagglutination activity on chicken,duck or goose sera at pH 7.2.The virus was tested using RT-PCR,and the positive PCR product sequenced and analyzed.The length of the amplified fragment was about 1kb.The sequenced nucleotide had the greatest homogeneity(i.e.,88.7%) with Tembusu Virus at the nucleotide position 9-777.Phylogenetic analysis of Flaviviridae showed the isolated WR strain appeared to share a common ancestor with Tembusu,Louis encephalitis,Israel turkey meningoencephalomyelitis and Ntaya Viruses.However,it seemed to have its own branch on the evolution tree.The results indicated that the isolated WR strain belonged to the genus Flavirirus,but not a member of any of the known groups in the genus.It might be a new Flavivirus species that has not been reported previously.

Key words: Laying ducks, egg-laying reduction, Flavivirus, new species, isolation and identification

中图分类号: 

  • S855.3