抗猪大肠杆菌功能性卵黄粉的制备及对小鼠的攻毒保护作用

福建农业学报 ›› 2011, Vol. ›› Issue (2): 159-165.

抗猪大肠杆菌功能性卵黄粉的制备及对小鼠的攻毒保护作用

杨金先¹, 郭庆², 俞伏松¹, 叶鼎承², 曾丽莉², 宋铁英¹

1. 福建省农业科学院生物技术研究所, 福建福州350003;
2. 福建省华龙集团饲料有限公司, 福建福州350003

收稿日期:2010-11-15 修回日期:2011-02-11 出版日期:2011-04-15 发布日期:2011-04-15
通讯作者: 宋铁英(1963-),女,研究员,主要从事动物病害防治(E-mail:tieyingsong@163.com)
基金资助:
科技部科技人员服务企业行动项目(2009GJC40042);科技部农业科技成果转化资金项目(2010GB2C400209);福建省科技计划重大专项(2010NZ0002-3)

Preparation of Egg Yolk Antibody Against Pig Pathogenic Escherichia coli and Its Passive Immunoprotection of Mouse from Challenge

YANG Jin-xian¹, GUO Qing², YU Fu-song¹, YE Ding-ceng², ZENG Li-li², SONG Tie-ying¹

1. Bio-technology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 35000;
2. Hualong Feedstuffs Technology and Development Group Company in Fujian Province, Fuzhou, Fujian 350003, China

Received:2010-11-15 Revised:2011-02-11 Online:2011-04-15 Published:2011-04-15

摘要/Abstract

摘要： 通过制备抗致病性大肠杆菌特异性IgY及富含特异性IgY的功能性卵黄粉,进行小鼠的攻毒保护试验,探讨功能性卵黄粉对由致病性大肠杆菌引起的疾病的防治作用。结果显示:(1)致病性大肠杆菌与特异性IgY混合后,菌体出现凝集,且病原菌的运动力明显受抑制;(2)浓度为1.3×10⁹CFU·mL^-1的致病性大肠杆菌K88、K99和987P攻毒小鼠后死亡率为60%,而将病原菌液与特异性IgY混合后攻毒小鼠,死亡率仅为10%;(3)饲喂功能性卵黄粉30 d后用浓度为2.6×10⁹CFU·mL^-1的致病性大肠杆菌K88、K99和987P攻毒小鼠的死亡率为40%,而同等剂量的对照攻毒小鼠死亡率为84%;并且饲喂功能性卵黄粉试验组小鼠的血清ALT、AST和ALP检测值显著低于对照组;肝、脾、胸腺的脏器指数、肠绒毛高度和V/C比值显著增高。试验结果说明:功能性卵黄粉中的特异性IgY可粘附并阻断致病性大肠杆菌对小鼠的致病力,减轻病原菌攻毒对小鼠内脏和肠道组织的功能损伤,并提高动物机体耐受由致病性大肠杆菌引起疾病的作用,从而促进动物肠道对营养物质的吸收,提高动物生长性能。

关键词: 功能性卵黄粉, 特异性IgY, 致病性大肠杆菌

Abstract: The experiments were conducted to investigate whether or not egg yolk antibodies can be used as a functional egg yolk powder preparation in the prevention and treatment for enteric infection caused by pathogenic Escherichia coli.The major results were as follows: 1) agglutination reaction appeared after the egg yolk antibodies mixed with the pathogenic Escherichia coli,and the bacteria activity decreased which's not agglutination;2) the mortality rate of mouse was 60% after challenge with 1.3×10⁹ CFU·mL^-1 Escherichia coli pathogen K88,K99 and 987P,while the mortality rate of mouse decreased to 10% after the pathogen mixed with egg yolk antibodies;3) after feeding functional egg yolk powder for 30 days and challenge with 2.6×10⁹ CFU·mL^-1,the mortality rate of mouse was 40% compared with the control group in 84%.Comparing with control group,the serum ALT,AST and ALP were significantly lower in treated mouse and the organ index of liver,spleen,thymus,the intestinal villus height and V/C ratio were significantly higher.The results indicated that the functional egg yolk powder can be prepared to prevent and treat the infection disease caused by pathogenic E.coli and effectively to improve the growth performance of mouse.

Key words: functional egg yolk powder, egg yolk antibodies, pathogenic Escherichia coli

中图分类号:

Q93-337

杨金先, 郭庆, 俞伏松, 叶鼎承, 曾丽莉, 宋铁英. 抗猪大肠杆菌功能性卵黄粉的制备及对小鼠的攻毒保护作用[J]. 福建农业学报, 2011, (2): 159-165.

YANG Jin-xian, GUO Qing, YU Fu-song, YE Ding-ceng, ZENG Li-li, SONG Tie-ying. Preparation of Egg Yolk Antibody Against Pig Pathogenic Escherichia coli and Its Passive Immunoprotection of Mouse from Challenge[J]. Fujian Journal of Agricultural Sciences, 2011, (2): 159-165.

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