1个水稻雌配子不育突变体的筛选及初步分析

福建农业学报 ›› 2011, Vol. 26 ›› Issue (4): 513-518.

1个水稻雌配子不育突变体的筛选及初步分析

颜静宛¹, 吴照祥^1,2, 刘华清¹, 王锋¹

1. 福建省农业科学院生物技术研究所, 福建福州 350003;
2. 福建农林大学生命科学学院, 福建福州 350002

收稿日期:2011-05-25 修回日期:2011-07-11 出版日期:2011-08-15 发布日期:2011-08-15
通讯作者: 王锋(1963-),男,博士,研究员,从事植物基因工程研究(E-mail: wf@fjage.org) E-mail:wf@fjage.org
作者简介:颜静宛(1977-),女,硕士,助理研究员,从事植物功能基因组研究
基金资助:
福建省自然科学基金(2008J0059)

Screening and Preliminary Analysis of A Female Gametophyte Sterile Mutant in Rice

YAN Jing-wan¹, WU Zhao-xiang^1,2, LIU Hua-qing¹, WANG Feng¹

1. Institute of Biological Technologis, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
2. College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

Received:2011-05-25 Revised:2011-07-11 Online:2011-08-15 Published:2011-08-15

摘要/Abstract

摘要： 在水稻转基因后代中,筛选到1个与雌配子育性相关的突变体,该突变体自交后代群体外源标记基因(潮霉素磷酸转移酶hpt)保持1∶1的分离规律,连续6代自交没有得到纯合株系;正、反杂交以及测交分析显示,潮霉素抗性基因只能通过雄配子进行传递;结实率统计分析表明具有外源标记基因hpt的单株有近乎一半的雌配子发生败育,胚囊的育性与外源基因hpt共分离;Southern-blot分析表明该突变体T-DNA是以单拷贝方式整合到水稻染色体上。这些分析表明该突变体是1个T-DNA插入导致的雌配子体不育突变体(暂定名为female gametogenesis sterile 1,fgs1)。细胞学观察显示,突变体fgs1花粉育性与野生型明恢86近似,但是其雌配子发育受阻,突变基因型雌配子体在8核胚囊时期,卵细胞、助细胞、反足细胞团以及中央极核依次解体退化,无法完成授粉受精。

关键词: 水稻, 雌配子不育突变体, 筛选, 胚囊败育, 激光扫描共聚焦显微镜

Abstract: We isolated a female gamete to a fertility-related mutant,from a T-DNA insertional population of indica rice.The transgenic plants carrying a T-DNA insertion displayed phenomena that hygromycin resistance and sensitivity segregation ratios showed 1∶1 by χ²-test and the homozygous progeny was not found in 6 successive generations.Reciprocal crosses between heterozygous plants and the wild types showed that the allele could not be transmitted through the female.Statistical analysis showed that the ripening rate of exogenous marker gene hpt with the plant has occurred almost half of the female gamete abortion,embryo sac fertility and closely linked to the hpt gene.Southern-blot showed that the mutant carried with a copy of T-DNA insertion.All of these indicated that the mutant is a gametophyte ovary sterile mutant caused by T-DNA insertion(tentatively named as female gametogenesis sterile 1,fgs1).Cytologic observation showed that pollen fertility of fgs1 was as normal as the wild type minghui 86,but the development of female gamete was blocked.In the female gamete of fgs1 mutant,we found that egg cell(E),synergids(S),antipodal cells(A)and polar nuclei(P)appeared disaggregation one by one during the 8 nucleuses period and the fertilization halted.

Key words: rice(Oryza sativa L.), female gametophyte sterile mutant, screening, embryo sac abortion, laser scanning confocal microscope(LSCM)

中图分类号:

S511

颜静宛, 吴照祥, 刘华清, 王锋. 1个水稻雌配子不育突变体的筛选及初步分析[J]. 福建农业学报, 2011, 26(4): 513-518.

YAN Jing-wan, WU Zhao-xiang, LIU Hua-qing, WANG Feng. Screening and Preliminary Analysis of A Female Gametophyte Sterile Mutant in Rice[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 513-518.

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