水稻斑点叶突变体W1764的遗传分析及初步定位

福建农业学报 ›› 2011, Vol. 26 ›› Issue (4): 519-522.

水稻斑点叶突变体W1764的遗传分析及初步定位

杨绍华, 刘华清, 王锋

福建省农业科学院生物技术研究所福建省农业遗传工程重点实验室, 福建福州 350003

收稿日期:2011-03-21 修回日期:2011-05-11 出版日期:2011-08-15 发布日期:2011-08-15
通讯作者: 王锋(1963-),男,博士,研究员,主要从事水稻基因工程研究(E-mail: wf@fjage.org) E-mail:wf@fjage.org
作者简介:杨绍华(1976-),男,博士,助理研究员,主要从事水稻功能基因研究
基金资助:
福建省财政专项——福建省农业科学院科技创新团队项目(CXTD2011-09)

Genetic Analysis and Gene Mapping of a Spotted Leaf Mutant W1764 in Rice

YANG Shao-hua, LIU Hua-qing, WANG Feng

Fujian Province Key Laboratory of Agricltural Genetic Engineering, Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

Received:2011-03-21 Revised:2011-05-11 Online:2011-08-15 Published:2011-08-15

摘要/Abstract

摘要： 从籼稻品种"明恢86"转基因后代中发现1个非T-DNA插入的叶斑突变体W1764。突变体在分蘖后期最先从基部叶叶尖处开始出现褐色斑点,随着植株的生长,斑点逐渐从叶尖扩展到整个叶片,同时从下到上叶片依次出现表型,至成熟时几乎遍布整个植株。遗传学分析表明,该突变体表型受单个隐性核基因控制。以突变体W1764和粳稻品种02428杂交构建F²群体,用其中突变表型的136株个体进行基因定位,将W1764的突变位点初步定位在第12染色体长臂的分子标记NSSR1229和L05-78之间1.9cM内。初步鉴定为1个新的叶斑突变基因,暂命名为spl30(t)。

关键词: 水稻, 叶斑突变, 遗传分析, 基因定位

Abstract: A non-T-DNA insertion spotted leaf mutant W1764 was identified in a transgenic offspring of rice(Oryza sativa L.)indica cv.Minghui86.The spot phenotype was first appearing at the tip part of the lowest leaf at late stage of tillering.As the plant growing,the spot spread from tip part to the whole leaves from lower leaves to the upper ones.When the mutant plant matured,the spot spread to the whole plant.Genetic analysis showed that the mutant phenotype was controlled by a single recessive gene.F² population build up with mutant W1764 and hybrid rice varieties 02428,and the 136 mutant phenotypes were used for gene targeting,we mapped the mutant gene in 1.9 cM interval between DNA marker NSSR1229 and L05-78 on the long arm of chromosome 12.We temporarily named the gene as spotted leaf 30(t)[spl30(t)].

Key words: Rice, Spotted leaf mutant, Genetic analysis, Gene mapping

中图分类号:

Q943.2

杨绍华, 刘华清, 王锋. 水稻斑点叶突变体W1764的遗传分析及初步定位[J]. 福建农业学报, 2011, 26(4): 519-522.

YANG Shao-hua, LIU Hua-qing, WANG Feng. Genetic Analysis and Gene Mapping of a Spotted Leaf Mutant W1764 in Rice[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 519-522.

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