猪繁殖与呼吸综合征(PRRS)抗体水平检测与分析

福建农业学报 ›› 2011, Vol. 26 ›› Issue (4): 552-556.

猪繁殖与呼吸综合征(PRRS)抗体水平检测与分析

曾显成¹, 陈晓实¹, 罗先¹, 吴发丹², 林乙铃², 陈家祥¹, 邵良平¹

1. 福建农林大学动物科学学院, 福建福州 350002;
2. 福建农林大学动物科学学院动物医院, 福建福州 350002

收稿日期:2011-05-22 修回日期:2011-06-30 出版日期:2011-08-15 发布日期:2011-08-15
通讯作者: 邵良平(1955-),男,教授,主要从事临床兽医学研究(E-mail: shaolp1955@126.com) E-mail:shaolp1955@126.com
作者简介:曾显成(1974-),男,实验师,硕士,主要从事预防兽医学研究(E-mail: zxc206@163.com)

Estimation of Antibody in Vaccinated and Non-vaccinated Pig Farms with PRRS Vaccine

ZENG Xian-cheng¹, CHEN Xiao-shi¹, LUO Xian¹, WU Fa-dan², LIN Yi-ling², CHEN Jia-xiang¹, SHAO Liang-ping¹

1. College of Animal Science, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China;
2. Animal Hospital, College of Animal Science, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China

Received:2011-05-22 Revised:2011-06-30 Online:2011-08-15 Published:2011-08-15

摘要/Abstract

摘要： 为探讨PRRS免疫猪场和非免疫猪场抗体水平差异,采集福建各地区64个不同规模猪场血清样本1 742份,其中免疫猪场40个共1 059份血清,非免疫猪场24个共683份血清;采用美国IDEXX公司PRRS病毒抗体ELISA试剂盒进行检测,分析其抗体阳性率、CV值及S/P区间分布值。结果显示:免疫猪场抗体阳性883份,阳性率算术平均值为83.4%,CV值为67.1,S/P值大于2.5的样本44份,占4.2%;非免疫猪场抗体阳性290份,阳性率算术平均值为42.5%,CV值为87.4,S/P值大于2.5的样本74份,占10.1%。表明免疫猪场抗体阳性率高,离散度小;非免疫猪场均可检测到PRRS阳性抗体,离散度大。揭示应加强PRRS的免疫,对疫情防控具有积极作用。

关键词: 猪繁殖与呼吸综合征, 抗体

Abstract: 1059 serum samples from 40 swine farms of vaccination with PRRSV vaccine and 683 serum samples from 24 swine farms of non-vaccination with PRRSV vaccine were collected to determine antibody level of PRRSV with IDEXX PRRSV antibody ELISA Kit.Positive rate,coefficient variation(CV)and Sample/Positive(S/P)were analyzed.The result showed that in vaccinated pig farms,883 of 1059 serum samples were positive of PRRSV antibody,average positive rate and CV were 83.4% and 67.1,respectively;S/P of 44 serum samples was higher than 2.5,accounting for 4.2%.In non-vaccinated pig farms,290 of 683 serum samples were positive of antibody of PRRSV,average positive rate and CV were 42.5% and 87.4,respectively;S/P of 74 serum samples was higher than 2.5,accounting for 10.1%.The results indicated that non-vaccinated pig farms have been infected by PRRSV.Average positive rate of PRRSV antibody is higher in vaccinated pig farms than in non-vaccinated pig farms.Simultaneously,CV and S/P in vaccinated pig farms decreased obviously compared to non-vaccinated pig farms.It is concluded that vaccination with PRRSV vaccine is one of better ways to control PRRS.

Key words: Porcine reproductive and respiratory syndrome(PRRS), Antibody

中图分类号:

S858.28

曾显成, 陈晓实, 罗先, 吴发丹, 林乙铃, 陈家祥, 邵良平. 猪繁殖与呼吸综合征(PRRS)抗体水平检测与分析[J]. 福建农业学报, 2011, 26(4): 552-556.

ZENG Xian-cheng, CHEN Xiao-shi, LUO Xian, WU Fa-dan, LIN Yi-ling, CHEN Jia-xiang, SHAO Liang-ping. Estimation of Antibody in Vaccinated and Non-vaccinated Pig Farms with PRRS Vaccine[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 552-556.

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