福建农业学报 ›› 2011, Vol. 26 ›› Issue (5): 818-821.

• 食品科学 • 上一篇    下一篇

木薯淀粉酶解糖化工艺研究

张树河, 李海明, 潘世明, 林一心   

  1. 福建省农业科学院甘蔗研究所,福建 漳州 363005
  • 收稿日期:2011-05-23 修回日期:2011-08-18 出版日期:2011-10-15 发布日期:2011-10-15
  • 通讯作者: 林一心(1962-),男,研究员,主要从事甘蔗选育种及能源植物转化制生物燃料的研究(E-mail:sugarcane888@sina.com) E-mail:sugarcane888@sina.com
  • 作者简介:张树河(1975-),男,助理研究员,主要从事能源植物的引进、栽培及转化制生物燃料的研究(E-mail:shuhe35@sina.com)
  • 基金资助:

    福建省科技计划项目——省属公益类科研院所基本科研专项(2011R1015-1);福建省财政专项——福建省农业科学院科技创新团队项目(STIF-Y05)

Saccharification of Cassava Starch with Enzymtic Catabolism

ZHANG Shu-he, LI Hai-ming, PAN Shi-ming, LIN Yi-xin   

  1. Sugarcane Research Institute,Fujian Academy of Agricultural Sciences,Zhangzhou,Fujian 363005,China
  • Received:2011-05-23 Revised:2011-08-18 Online:2011-10-15 Published:2011-10-15

摘要: 以淀粉的水解程度(DE值)为指标,对木薯淀粉糖化条件进行了研究,分析了糖化温度、时间、酶用量、pH和转速等单因素范围分别对淀粉水解的影响,结果表明:糖化温度在60~65℃,反应时间在105~115 min,酶用量在1.5~2.5 mL,pH在3.5~4.5,转速约为180 r·min-1时淀粉水解的效果最好。通过正交试验设计确定了最佳糖化条件:糖化温度65℃、糖化时间105 min、酶用量2 mL·L-1、pH 4.0,糖化的DE值达到105%。

关键词: 木薯, 淀粉, 糖化

Abstract: The saccharification of cassava starch was studied by using dextrose equivalent as index. The effect of single factor was analyzed in the best efficiency of saccharification. Results showed that the proper value of the factor were for temperature 60 to 65℃, reaction time 105 to 115 min, enzyme level 1.5 to 2.5 mL, pH 3.5 to 4.5 and rotation rate in 180 rpm, respectively. However, the optimum parameters for saccharification were determined by orthogonal experiments as pH at 4.0, with enzyme of 2 mL·L-1 and in which treated for 105 min at 65℃. Saccharification efficiency (DE) could reach to 105%.

Key words: cassava, starch, saccharification

中图分类号: 

  • TS 231