棉籽蛋白水解物水解度3种测定方法的比较

福建农业学报 ›› 2011, Vol. 26 ›› Issue (6): 1076-1080.

棉籽蛋白水解物水解度3种测定方法的比较

林虬^1,2, 黄薇^1,2, 宋永康^1,2, 姚清华^1,2, 颜孙安^1,2, 钱爱萍^1,2

1. 福建省农业科学院中心实验室,福建福州 350003;
2. 福建省精密仪器农业测试重点实验室,福建福州 350003

收稿日期:2011-05-26 修回日期:2011-09-12 出版日期:2011-12-15 发布日期:2011-12-15
作者简介:林虬(1963-),男,副研究员,主要从事水产饲料研究(E-mail:linqiu3163@yahoo.com.cn)
基金资助:
福建省科技计划项目——省属公益类科研院所基本科研专项(2010R1027-1);福建省科技计划重大专项(2010NZ0002);福建省农业科学院青年人才创新基金(2010QC-17)

Comparing Three Methods in Determining Hydrolytic Degree of Cottonseed Protein

LIN Qiu^1,2, HUANG Wei^1,2, SONG Ying-kang^1,2, YAO Qing-hua^1,2, YAN Sun-an^1,2, QIAN Ai-ping^1,2

1. Central Laboratory, Fujian Academy of Agricultural Sciences, Fuzhou,Fujian 350003,china;
2. Fujian Key Laboratory of Precision Measurement of Agricultural Sciences, Fuzhou,Fujian 350003

Received:2011-05-26 Revised:2011-09-12 Online:2011-12-15 Published:2011-12-15

摘要/Abstract

摘要： 介绍了一种比较简单可行、适用于棉籽蛋白水解度的测定方法——OPA法。选用中性蛋白酶将棉籽蛋白水解成棉籽多肽,通过新建立的方法测定不同酶解时间棉籽蛋白水解液的水解度,并与文献所报道的甲醛滴定法和茚三酮比色法进行比较。结果表明,与甲醛滴定法和茚三酮比色法相比,OPA法更简便、更快速,且灵敏度高、可重复性强、对环境友好,是3种方法中最适合测定棉籽蛋白水解液水解度的方法,同时该方法也适用于饲料及食品行业中其他蛋白水解度的测定。

关键词: 水解度, 棉籽蛋白, 对苯二甲醛法

Abstract: This paper introduced a method that based on the reaction of primary amino groups with o-phthaldialdehyde (OPA) for determining the hydrolytic degree of cottonseed protein.Neutral protease was used to hydrolyze cottonseed protein and the degree of hydrolysis (DH) was compared with three assay methods, i.e.o-phthaldialdehyde method, formaldehyde titration method and ninhydrin colorimetric method.Comparing with formaldehyde titration and ninhydrin colorimetric methods, results showed that OPA method performed more accurate and faster in analyzing, easier to handle, repeatable in results and friendly for environment.It is recommended that OPA method is an appropriate method for evaluating in hydrolytic degree of cottonseed protein.

Key words: hydrolytic degree, cottonseed protein, o-phthaldialdehyde method

林虬, 黄薇, 宋永康, 姚清华, 颜孙安, 钱爱萍. 棉籽蛋白水解物水解度3种测定方法的比较[J]. 福建农业学报, 2011, 26(6): 1076-1080.

LIN Qiu, HUANG Wei, SONG Ying-kang, YAO Qing-hua, YAN Sun-an, QIAN Ai-ping. Comparing Three Methods in Determining Hydrolytic Degree of Cottonseed Protein[J]. Fujian Journal of Agricultural Sciences, 2011, 26(6): 1076-1080.