紫云英根瘤菌的分离与鉴定

福建农业学报 ›› 2012, Vol. ›› Issue (5): 524-532.

紫云英根瘤菌的分离与鉴定

管凤贞^1,2, 钟少杰³, 邱宏端², 林戎斌¹, 张辉¹, 陈济琛¹, 林新坚¹

1. 福建省农业科学院土壤肥料研究所, 福建福州 350003;
2. 福州大学生物科学与工程学院, 福建福州 350108;
3. 福建农林大学生命科学院, 福建福州 350002

收稿日期:2012-03-22 修回日期:2012-05-01 出版日期:2012-05-30 发布日期:2012-05-30
通讯作者: 林新坚(1955- ),男,研究员,研究方向:微生物与土壤培肥(E-mail:xinjianlin@163.net)
基金资助:
福建省科技计划项目——省属公益类科研院所基本科研专项(2010R10245)

Isolating and Identification of Rhizobial strains of Chinese Milk Vetch

GUAN Feng-zhen^1,2, ZHONG Shao-jie³, QIU Hong-duan², LIN Rong-bin¹, ZHANG Hui¹, CHEN Ji-chen¹, LIN Xin-jian¹

1. Soil and Fertilizer Institute of Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
2. Biological Science and Engineering Department of Fuzhou University, Fuzhou, Fujian 350108, China;
3. Life science department of Agricultural University of Fujian, Fuzhou, Fujian 350002, China

Received:2012-03-22 Revised:2012-05-01 Online:2012-05-30 Published:2012-05-30

摘要/Abstract

摘要： 从采自福建各地紫云英的根瘤中分离出具有一般根瘤菌特征的菌株18株,在对其进行初步细菌学特性鉴定的基础上,利用16S rDNA进行序列分析,确定其系统发育地位。鉴定结果为:YX、Z3、GZ与ZK1为中慢生根瘤菌属Mesorhizobium菌种,B3、XZ、M6与百脉根中生根瘤菌Mesorhizobiumloti可能为同一菌属的不同变种;ZLH2为黄单孢菌属Xanthomonas菌种;M3、M10、ZK7、ZQH、B4、B7为土壤杆菌属Agrobacterium菌种;B6、B5、B1、 Z1为根瘤菌属Rhizobium菌种。为了进一步考察各鉴定菌株的结瘤情况,利用琼脂试管法和水培法将部分菌株与紫云英闽紫品种(1号、5号、6号、7号)和80410411品系进行配对结瘤,研究结果不仅进一步阐明了紫云英根瘤菌的系统发育多样性,并且通过对比琼脂试管法和水培法在结瘤结果上的异同,为探究紫云英与不同根瘤菌结瘤能力的差异提供了一种简便可行的研究方法。

关键词: 紫云英根瘤菌, 分离, 16S rDNA, 鉴定, 琼脂试管法, 水培法, 结瘤

Abstract: A total of 18 isolates with general characteristics of Rhizobium were isolated from root nodules of Chinese milk vetch from different areas of Fujian. 16S rDNA sequence analysis was conducted on these bacteria with the purpose of determine the phylogenetic analyses. The result showed that YX、Z3、GZ and ZK1 were Mesorhizobium; B3、XZ、M6 were Mesorhizobium loti or varieties, ZLH2 was Xanthomonas, M3、M10、B4、B7、ZK7 and ZQH were Agrobacterium, B6、B5、B1 and Z1 were Rhizobium. For the purpose of investigate the nodulation performance of the identified bacterial, water culture and agar tube culture were applied between the part of identified rhizobial striains and milk vetch Minzi 1、Minzi 5、Minzi 6、Minzi 7 and 80410411. This study not only further elucidate the diversity of Chinese milk vetch rhizobium, but also provides a simple and feasible method to explore the differences in the ability of different rhizobium nodulation by contrast the different of nodulation with the agar tube culture and water culture.

Key words: Chinese milk vetch rhizobium, separate, 16S rDNA, identification, agar tube culture, water culture, nodulation

中图分类号:

S144.3

管凤贞, 钟少杰, 邱宏端, 林戎斌, 张辉, 陈济琛, 林新坚. 紫云英根瘤菌的分离与鉴定[J]. 福建农业学报, 2012, (5): 524-532.

GUAN Feng-zhen, ZHONG Shao-jie, QIU Hong-duan, LIN Rong-bin, ZHANG Hui, CHEN Ji-chen, LIN Xin-jian. Isolating and Identification of Rhizobial strains of Chinese Milk Vetch[J]. Fujian Journal of Agricultural Sciences, 2012, (5): 524-532.

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