开菲尔粒中醋酸菌的分离鉴定

福建农业学报 ›› 2012, Vol. ›› Issue (5): 544-549.

开菲尔粒中醋酸菌的分离鉴定

刘芸, 曹宜, 刘波, 刘丹莹, 朱育菁, 刘欣

福建省农业科学院农业生物资源研究所, 福建福州 350003

收稿日期:2012-02-03 修回日期:2012-05-02 出版日期:2012-05-30 发布日期:2012-05-30
通讯作者: 刘波(1957- ),男,博士,研究员,主要从事生物技术和生物防治研究(E-mail:fzliubo@163.com)
基金资助:
福建省科技计划项目——省属公益类科研院所基本科研专项(2011R1020-7)

Isolation and Identification of Acetobacter Bacteria from Kefir Grains

LIU Yun, CAO Yi, LIU Bo, LIU Dan-ying, ZHU Yu-jing, LIU Xin

Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

Received:2012-02-03 Revised:2012-05-02 Online:2012-05-30 Published:2012-05-30

摘要/Abstract

摘要： 从开菲尔粒中分离出2株醋酸菌FJAT-13764和FJAT-13780。利用形态学观察、生理生化和16S rDNA方法对菌株进行分类鉴定。菌株FJAT-13764和FJAT-13780的菌体形态均为圆端直杆菌,单个或成对,无芽孢,其生理生化特征与醋杆菌属Acetobacter基本一致。经16S rDNA基因序列同源性比较和系统发育分析,鉴定菌株FJAT-13764为Acetobacter orientalis(东方醋酸菌),菌株FJAT-13780为Acetobacter syzygii。

关键词: 开菲尔, 鉴定, 醋酸杆菌, 东方醋酸菌, Acetobacter syzygii

Abstract: Two Acetobacter strains FJAT-13764 and FJAT-13780 were isolated from kefir grains. The observations with scanning electron microscope showed that both bacteria strains were rod-shaded with round terminals, presented in single or pairs,produced no spores. Their orerall biochemical and physiological characters indicated that these strains should be placed in the Acetobacter spp. The 16S rDNA sequence analysis indicated that FJAT-13764 and FJAT-13780 were Acetobacter orientalis and Acetobacter syzygii, respectively. This study is the first report in Acetobacter orientalis isolated from kefir grains,which provides the basic information for further researches on the micro-flora diversity in kefir grain.

Key words: kefir grains, identification, Acetobacter bacteria, Acetobacter orientalis, Acetobacter syzygii

中图分类号:

Q93-331

刘芸, 曹宜, 刘波, 刘丹莹, 朱育菁, 刘欣. 开菲尔粒中醋酸菌的分离鉴定[J]. 福建农业学报, 2012, (5): 544-549.

LIU Yun, CAO Yi, LIU Bo, LIU Dan-ying, ZHU Yu-jing, LIU Xin. Isolation and Identification of Acetobacter Bacteria from Kefir Grains[J]. Fujian Journal of Agricultural Sciences, 2012, (5): 544-549.

参考文献

[1] 袁勇军,黄丽金,陈伟. 发酵乳饮料——开菲尔的研究进展 [J]. 安徽农业科学, 2009, 37(23):11154-11156.
[2] WITTHUHN R C, SCHOEMAN T, BRITZ T J, et al. Characterisation of the microbial population at different stages of kefir production and kefir grain mass cultivation [J]. International Dairy Journal, 2005, 15:383-389.
[3] GARROTE G L, ABRAHAM A G, DE ANTONI G L. Chemical and microbiological characterization of kefir grain [J]. Journal of Dairy Research, 2001, 68(4):639-652.
[4] 王亚峰,霍贵成,刘丽波. 开菲尔粒中乳酸菌的分离与鉴定 [J]. 中国乳品工业,2004, 32(9):17-19.
[5] 王和平,王锂韫,李少刚,等. 类开菲尔粒中乳酸菌和酵母菌的分离鉴定及生物学特性 [J]. 中国乳品工业,2004, 32(12):3-6.
[6] 刘慧,李平兰,官品,等. 开菲尔粒中酵母菌的分离及其发酵性能的研究 [J]. 食品科学,2005, 26(2):97-100.
[7] 周剑忠,黄开红,董明盛,等. 开菲尔粒和西藏灵菇中细菌和酵母群落结构的比较 [J]. 江苏农业学报,2008, 24(5):634-639.
[8] HONG W S, CHEN H S, CHEN Y P, et al. Effect of kefir supernatant and lactic acid bacteria isolated from kefir grain on cytokine production by macrophage [J]. International dairy Journal, 2009, 19:244-251.
[9] 杨远剑. 开菲尔微生态的研究进展 [J]. 肉类研究, 2008,(5):29-33.
[10] 林晓珊,吴虹,杨汝德. 中华开菲尔共生菌的分离与鉴定 [J]. 现代食品科技, 2009, 25(10):1233-1235.
[11] 中华人民共和国国家标准. GB/T 4789.35-2003 食品卫生微生物检验食品中乳酸菌检验 [S]. 北京:中国标准出版社,1996:273-280.
[12] 张和平. 一种适合乳酸菌16S rRNA序列测定的引物设计:中国,200910250674.6.2010-05-12.
[13] BUCHANAN R E,GIBBONE E N. 伯杰氏细菌鉴定手册 [M]. 中国科学科学院微生物研究所,译. 第8版. 北京:科学出版社,1984:362-366.
[14] 东秀珠,蔡妙英. 常见细菌系统鉴定手册 [M]. 北京:科学出版社,2001:135-137.
[15] 陈卫红, 沈伟达,阳大海,等. 开菲尔粒中一株醋酸菌的分离鉴定[J]. 安徽农业科学, 2010, 38(21):11045-11046.
[16] 余世琳. 古老而新型的酒精性发酵乳饮料——开菲尔 [J]. 中国乳品工业, 1999, 27(2):18-23.
[17] 余东霞. 一种新型发酵乳——Kefir [J]. 食品工业, 1999(1):25-26.
[18] FARNWORTH E R. Kefir from folklore to regulatory approveal [J]. Journal of Nutraceuticals, Functional & Medical Food, 1999, 1(14):57-68.
[19] 曾友明,曾文兵,冯司亮,等. 开菲尔功能菌组的复合及发酵特性研究 [J]. 中国乳品工业, 2007, 35(3):26-28.
[20] MIGUEL M G C P, CARDOSO P G, LAGO L A. Diversity of bacteria present in milk kefir, grains using culture-dependent and culture-independent methods[J]. Food Research International, 2010, 43(5):1523-1528.
[21] 夏菠,张庆,罗阳,等. 开菲尔粒及其发酵液中菌相分析 [J]. 食品与机械, 2004, 20(6):13-14.
[22] 夏菠,周传云,张庆,等. 开菲尔中菌种分离与鉴定方法初探 [J]. 现代食品科技, 2005, 21(4):70-72.
[23] 张丽霞,黄开红,周剑忠. 国外开菲尔粒中乳酸菌的分离纯化与鉴定研究 [J]. 江西农业学报,2008, 20(5):85-87.
[24] 张国亮,冀朵朵,金苏,等. 开菲尔粒菌相构成的研究 [J]. 乳业科学与技术,2009, 3:110-112.
[25] ZHOU J Z, LIU X L, JIANG H H, et al. Analysis of the microflora in Tibetan kefir grains using denaturing gradient gel electrophoresis[J]. Food Microbiology, 2009, 26(8):770-775.
[26] 阳大海,陈卫红,沈伟达,等. 开菲尔粒中优势菌的筛选及鉴定 [J]. 安徽农业科学, 2010, 38(6):3146-3148.
[27] GARBERS I M, BRITZ T J, WITTHUHN R C. PCR-based denaturing gradient gel electrophoretic typification and identification of the microbial consortium present in kefir grain [J]. World Journal of Microbiology & Biotechnology, 2004, 20:687-693.