Cre/loxp位点特异性重组系统在转基因植物中的应用

福建农业学报 ›› 2008, Vol. ›› Issue (2): 211-217.

Cre/loxp位点特异性重组系统在转基因植物中的应用

邱淑萍^1,2, 陈在杰¹, 王锋¹

1. 福建省农业科学院福建农业遗传工程重点实验室, 福建福州, 350003;
2. 福建师范大学生命科学学院, 福建福州, 350007

收稿日期:2008-03-25 修回日期:2008-05-28 出版日期:2008-04-15 发布日期:2008-04-15
通讯作者: 王锋(1963- ),男,博士,研究员,从事植物基因工程研究(E-mail:wf@fjage.org)
基金资助:
福建省自然科学基金(B01220003)

Application of Cre/loxp site-specific recombination technology on transgenic plants

QIU Shu-ping^1,2, CHEN Zai-jie¹, WANG Feng¹

1. Fujian Key Laboratory of Genetic Engineering for Agriculture, Fujian Academy of Agricul-tural Sciences, Fuzhou, Fujian 350003, China;
2. College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350007, China

Received:2008-03-25 Revised:2008-05-28 Online:2008-04-15 Published:2008-04-15

摘要/Abstract

摘要： 位点特异性重组系统能够在植物遗传转化中更准确、可靠的操纵外源DNA的引入或删除,已成为植物遗传操作中的重要工具。本文简要介绍目前应用最为广泛的Cre/loxp位点特异性重组系统的重组机制,并着重阐述Cre/loxp系统在删除转基因植物中标记基因及定点整合等方面的应用。

关键词: 位点特异性重组, Cre/loxp, 转基因植物, 基因删除, 定点整合

Abstract: Site-specific recombination technology has been developed and widely applied for plant genetic manipulations.It can successfully delete marker genes from a transgenic plant or site-specifically integrate a gene to a pre-determined genomic location in a precise,single-copy pattern.This article reviews the Cre/loxp site-specific recombination technique and its applications for marker gene deletion and precise gene integration.

Key words: site-specific recombination, Cre/loxp, transgenic plant, gene deletion, site-specific integration

中图分类号:

Q943.2

邱淑萍, 陈在杰, 王锋. Cre/loxp位点特异性重组系统在转基因植物中的应用[J]. 福建农业学报, 2008, (2): 211-217.

QIU Shu-ping, CHEN Zai-jie, WANG Feng. Application of Cre/loxp site-specific recombination technology on transgenic plants[J]. Fujian Journal of Agricultural Sciences, 2008, (2): 211-217.

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