羊栖菜提取物对食用菌竞争性杂菌生长的抑制作用

福建农业学报 ›› 2008, Vol. ›› Issue (3): 270-273.

羊栖菜提取物对食用菌竞争性杂菌生长的抑制作用

刘艳如¹, 王缨², 林勇³, 郑怡¹, 欧阳桐娇², 江枝和³, 郭文杰²

1. 福建师范大学生命科学学院, 福建, 福州, 350108;
2. 福建省农业科学院生物技术研究所, 福建, 福州, 350013;
3. 福建省农业科学院土壤肥料研究所, 福建, 福州, 350013

收稿日期:2007-07-26 修回日期:2008-05-09 出版日期:2008-07-15 发布日期:2008-07-15
基金资助:
福建省科技重点项目(2005N036)

Inhibition effect of Sargassum fusiforme extracts on pathogenic fungi

LIU Yan-ru¹, WANG Ying², LIN Yong³, ZHENG Yi¹, OU YANG Tong-jiao², JIANG Zhi-he³, GUO Wen-jie²

1. College Life Sciences, Fujian Normal University, Fuzhou, Fujian 350007, China; 2.Institute of Biotechnology Research, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
3. Institute of Soil and Fertilizer Research, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

Received:2007-07-26 Revised:2008-05-09 Online:2008-07-15 Published:2008-07-15

摘要/Abstract

摘要： 采用生长速率法和孢子萌发法对羊栖菜提取物的抗真菌活性进行了筛选,结果表明:R_f0.47(10g•kg^-1、1 g•kg^-1)和R_f0.81(100 g•kg^-1、1 g•kg^-1)对圆弧青霉菌丝生长抑制率分别为35%、50%和85%、25%,羊栖菜乙醚提取物乙酸乙酯相的薄层分离带R_f0.30(100 g•kg^-1)对绿色木霉及黑曲霉抑制率分别为75.67%、83.46%。相关系数分析显示:不同浓度的3个处理组存在极显著的正相关。双因素方差分析表明:分离带和浓度两因素对绿色木霉、圆弧青霉、黑曲霉菌丝生长抑制率的影响极显著。在3种质量浓度下,羊栖菜乙醇R_f0.47、R_f0.81、羊栖菜乙醚R_f0.00对圆弧青霉孢子萌发以及羊栖菜乙醇乙酸乙酯相、羊栖菜乙醚乙酸乙酯相、羊栖菜乙醚R_f0.30对黑曲霉孢子萌发均有显著的抑制作用。

关键词: 羊栖菜, 抗菌活性, 薄层层析(TLC), 生长速率法, 孢子萌发法

Abstract: Anti-fungal activities of the extracts from Sargassum fusiforme were tested by means of hypha growth rate and spore germination.The results indicated that the R_f0.47 at 10 g•kg^-1,1 g•kg^-1 and R_f0.47 at 100 g•kg^-1,1 g•kg^-1 of the ethyl acetate phase on TLC from the S.fusiforme soaked in ethanol significantly inhibited Penicillium cyclopium growth.The inhibition rates of those components were 35%,50%,85% and 25%,respectively.The R_f0.30 at 100 g•kg^-1 of the same extract significantly inhibited Trichoderma viride and Aspergillus niger V.Tiegh growth with inhibition rates of 75.67% and 83.46%,respectively.The correlation analysis showed that concentrations of the 3 treatment groups significantly correlated to the inhibitory effect.Two-factors analysis of variance showed that the separation and concentration affected significantly on T.viride,P.cyclopium,A.niger V.Tiegh mycelial growth inhibition.In different concentrations,R_f0.47,R_f0.81 of Sargassum fusiforme soaked with ethanol,R_f0.00 of Sargassum fusiforme soaked with ether and ethanol ethyl acetate phase and ethyl acetate phase of Sargassum fusiforme ether,R_f0.30 of Sargassum fusiforme soaked with ether have the extremely significant inhitition on the germination of spores of Aspergillus niger V.Tiegh.

Key words: Sargassum fusiforme, anti-microbial activity, thin layer chromatography(TLC), growth rate method, spore germination method

中图分类号:

TS202

刘艳如, 王缨, 林勇, 郑怡, 欧阳桐娇, 江枝和, 郭文杰. 羊栖菜提取物对食用菌竞争性杂菌生长的抑制作用[J]. 福建农业学报, 2008, (3): 270-273.

LIU Yan-ru, WANG Ying, LIN Yong, ZHENG Yi, OU YANG Tong-jiao, JIANG Zhi-he, GUO Wen-jie. Inhibition effect of Sargassum fusiforme extracts on pathogenic fungi[J]. Fujian Journal of Agricultural Sciences, 2008, (3): 270-273.

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