鸡新城疫病毒F48E9强毒株NP基因的序列分析

福建农业学报 ›› 2009, Vol. ›› Issue (5): 385-389.

• 动物科学 • 下一篇

鸡新城疫病毒F48E9强毒株NP基因的序列分析

陈珍¹, 施少华^1,2, 程龙飞^1,2, 傅光华^1,2, 陈红梅¹, 万春和¹, 林芳¹, 林建生¹, 黄瑜^1,2

1. 福建省农业科学院畜牧兽医研究所, 福建, 福州, 350013;
2. 福建省畜禽疫病防治工程技术研究中心, 福建, 福州, 350013

收稿日期:2009-07-08 修回日期:2009-09-23 出版日期:2009-10-15 发布日期:2009-10-15
通讯作者: 黄瑜(1965- ), 男,博士,研究员,从事动物传染病研究
基金资助:
国家自然科学基金资助项目(30671564);国家“973”子项目(2005CB523001)

Sequence analysis on nucleocapsid protein gene of velogenic chicken-origin Newcastle disease virus,F48E9 strain

CHEN Zhen¹, SHI Shao-hua^1,2, CHENG Long-fei^1,2, FU Guang-hua^1,2, Chen Hong-mei¹, WAN Chun-he¹, LIN Fang¹, LIN Jian-sheng¹, HUANG Yu^1,2

1. Institute of Animal Husbandry and Veterinary, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2. Fujian Animal Diseases Control Techology Development Center, Fuzhou, Fujian 350013, China

Received:2009-07-08 Revised:2009-09-23 Online:2009-10-15 Published:2009-10-15

摘要/Abstract

摘要： 根据GenBank登录的鸡新城疫病毒NP基因序列设计引物,利用RT-PCR对F48E9强毒株NP基因进行扩增,将扩增产物经纯化后连入pMD18-T载体,通过PCR和酶切鉴定出阳性重组质粒,经测序后获得F48E9 NP基因序列。经分析发现该基因的ORF总长为1 470 bp,编码489 aa,NP蛋白有4个高度保守的Cys位点,分别位于第55、78、139、213位,且在401～440位和461～481位区域变异较大。将F48E9株与19株参考毒株的相应序列进行比较得到核苷酸序列同源性为87.9%～92.3%,氨基酸序列同源性较高,为92.2%～98.0%,遗传进化分析表明F48E9株相对独立,与参考毒株的遗传距离较远。

关键词: 鸡新城疫病毒, F48E9株, NP基因, 序列分析

Abstract: The nucleocapsid protein gene of velogenic,chicken-origin,Newcastle disease virus(NDV) F48E9 was amplified by RT-PCR based on a specific pair of primers designed according to the chicken-origin NDVs NP gene sequences published at GenBank.The purified RT-PCR products were cloned onto the pMD18-T vectors,and then,the positive recombinants had been identified by PCR and enzyme digestion.The sequencing results indicated that the F48E9 NP gene had an opening read frame of 1470nt in length with a coded polypeptide of 489 amino acids. There were four highly conserved cysteines on the F48E9 strain nucleocapsid protein.The amino acids at the regions, aa 401-440 and aa 461-481,were found to vary more than the others.The sequence analysis indicated that the F48E9 strain had a nucleotide homology of 87.9%-92.3%,and the amino acid homology of 92.2%-98.0% with other NDVs.The F48E9 strain appeared to be evolutionarily independent,with only remote relationship with other NDVs.

Key words: Newcastle disease virus, F48E9 strain, nucleocapsid protein gene, cloning

中图分类号:

陈珍, 施少华, 程龙飞, 傅光华, 陈红梅, 万春和, 林芳, 林建生, 黄瑜. 鸡新城疫病毒F48E9强毒株NP基因的序列分析[J]. 福建农业学报, 2009, (5): 385-389.

CHEN Zhen, SHI Shao-hua, CHENG Long-fei, FU Guang-hua, Chen Hong-mei, WAN Chun-he, LIN Fang, LIN Jian-sheng, HUANG Yu. Sequence analysis on nucleocapsid protein gene of velogenic chicken-origin Newcastle disease virus,F48E9 strain[J]. Fujian Journal of Agricultural Sciences, 2009, (5): 385-389.

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鸡新城疫病毒F48E9强毒株NP基因的序列分析

Sequence analysis on nucleocapsid protein gene of velogenic chicken-origin Newcastle disease virus,F48E9 strain

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