西瓜尖孢镰刀菌FOV-135的绿色荧光蛋白基因转化

福建农业学报 ›› 2009, Vol. ›› Issue (6): 521-524.

西瓜尖孢镰刀菌FOV-135的绿色荧光蛋白基因转化

肖荣凤, 朱育菁, 李燕丹, 黄素芳, 刘波

福建省农业科学院农业生物资源研究所, 福建, 福州, 350003

收稿日期:2009-11-28 修回日期:2009-12-31 出版日期:2009-12-15 发布日期:2009-12-15
通讯作者: 刘波(1957- ),男,研究员,从事农业生物技术研究(E-mail:zzx64@sohu.com)
基金资助:
福建省自然科学基金项目(2008J0055);福建省科技计划重点项目(2008N0020);福建省公益类科研院所基本科研专项(200903049);福建省农业科学院支撑计划项目(zcmn0801)

Green fluorescent protein gene transformation on Fusarium oxysporum f. sp. niveum strain, FOV-135

XIAO Rong-feng, ZHU Yu-jing, LI Yan-dan, HUANG Su-fang, LIU Bo

Institute of Agrobiological Resouces, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

Received:2009-11-28 Revised:2009-12-31 Online:2009-12-15 Published:2009-12-15

摘要/Abstract

摘要： 建立并优化了PEG-CaCl₂介导的原生质体转化体系,成功地将绿色荧光蛋白基因转入到西瓜尖孢镰刀菌FOV-135菌株中。转化子连续转接5代能够稳定遗传,荧光强度良好,每微克质粒DNA可获得4～6个性状稳定的转化子。PCR验证也表明gfp基因已转入到菌株FOV-135中。

关键词: 尖孢镰刀菌西瓜专化型, 绿色荧光蛋白, 原生质体转化

Abstract: The gene coding for green fluorescent protein(GFP) was successfully transferred to Fusarium oxysporum f.sp.niveum strain,FOV-135,by using the protoplast-mediated genetic transformation system.The transformants were stable after five successive subcultures.Four to six stable transformants could be obtained from one microgram of plasmid DNA.The PCR amplification confirmed that the gfp gene had been transformed to FOV-135.

Key words: Fusarium oxysporum f.sp.niveum, green fluorescent protein, protoplast-mediated genetic transformation system

中图分类号:

S432.44

肖荣凤, 朱育菁, 李燕丹, 黄素芳, 刘波. 西瓜尖孢镰刀菌FOV-135的绿色荧光蛋白基因转化[J]. 福建农业学报, 2009, (6): 521-524.

XIAO Rong-feng, ZHU Yu-jing, LI Yan-dan, HUANG Su-fang, LIU Bo. Green fluorescent protein gene transformation on Fusarium oxysporum f. sp. niveum strain, FOV-135[J]. Fujian Journal of Agricultural Sciences, 2009, (6): 521-524.

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