福建农业学报 ›› 2010, Vol. ›› Issue (5): 602-605.

• 园艺科学 • 上一篇    下一篇

铁皮石斛试管苗生根影响因素研究

陈青青1,2, 赖钟雄1,2, 朱金秀1,2   

  1. 1. 福建农林大学园艺学院, 福建, 福州 350002;
    2. 福建农林大学园艺植物生物工程研究所, 福建, 福州 350002
  • 收稿日期:2010-07-31 修回日期:2010-09-14 出版日期:2010-10-15 发布日期:2010-10-15
  • 通讯作者: 赖钟雄(1966- ),男,博士,研究员,主要从事园艺植物生物技术与遗传资源研究(E-mail:Laizx01@163.com)
  • 基金资助:
    福建省教育厅科技项目(JA09099)

Factors affecting plantlet rooting from Dendrobium officinale tissue culture

CHEN Qing-qing1,2, LAI Zhong-xiong1,2, ZHU Jin-xiu1,2   

  1. 1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
    2. Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
  • Received:2010-07-31 Revised:2010-09-14 Online:2010-10-15 Published:2010-10-15

摘要: 以铁皮石斛试管丛生苗为原料,研究不同pH值、光照强度和温度对铁皮石斛苗长势和生根的影响。结果表明:温度和光照对铁皮石斛苗株高、生根率和苗鲜重影响显著(P<0.05),而pH对铁皮石斛的苗株高影响不显著(P>0.05),对苗鲜重和生根率影响显著;铁皮石斛丛生苗壮苗生根的适宜pH值为5.4,适宜温度为25℃,光照强度为1500lx。

关键词: 铁皮石斛, 组培, 壮苗生根, 影响因素

Abstract: Effects of pH,light intensity and temperature on the growth and root formation of Dendrobium officinale cultivated in vitro were studied.Results showed that the height,rate of root formation and fresh weight of D.officinale were affected significantly by light intensity and temperature(P<0.05);and that the root formation and fresh weight were significant affected by pH(P<0.05),but not on the plantlet height.The optimal conditions for the growth and root formation of D.officinale were: pH 5.4 at 25℃ with the light intensity of 1500 lx.

Key words: Dendrobium officinale, tissue culture, growth, rooting

中图分类号: 

  • S567.23