水稻生殖发育突变体fro1(t)的形态发生及基因定位

福建农业学报 ›› 2010, Vol. ›› Issue (6): 677-683.

水稻生殖发育突变体fro1(t)的形态发生及基因定位

陈在杰, 吴煜兵, 陈少游, 刘华清, 王锋

福建省农业科学院农业遗传工程重点实验室, 福建福州, 350003

收稿日期:2010-10-14 修回日期:2010-12-01 出版日期:2010-12-15 发布日期:2010-12-15
通讯作者: 王锋(1963- ),男,博士,研究员,主要从事植物基因工程研究(E-mail:wf@fjage.org)
基金资助:
福建省自然科学基金(2007J0056)

Morphogenesis and gene-mapping of a reproducetive development mutant fro1(t) in rice

CHEN Zai-jie, WU Yi-bing, CHEN Shao-you, LIU Hua-qing, WANG Feng

Fujian Provincial Key Laboratory of Genetic Engineering for Agriculture, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

Received:2010-10-14 Revised:2010-12-01 Online:2010-12-15 Published:2010-12-15

摘要/Abstract

摘要： 水稻的花发育是稻米形成的基础,研究水稻花发育的机制具有十分重要的意义。在水稻组培后代中发现一个生殖发育突变体,暂命名为function of reproductive organ(fro1(t)),主要性状为内、外稃片扭曲变形抱合,小穗外形酷似辣椒,但小穗始终闭合不开花;副颖多数退化;浆片转变成薄叶状;雄蕊花丝膨大扭曲,花药表面扭曲变形,无花粉,雄蕊个数2～6枚,部分雄蕊转化为底部为叶状,顶部有类似柱头的雌蕊状器官;子房发育正常,但是多数花柱融合,柱头簇生,3～4个。扫描电镜结果显示,决定fro1(t)性状的基因调控着水稻花分生组织的分化及花器官决定的特化。利用图位克隆等技术对基因进行定位,最终将该突变位点定位在水稻8号染色体长臂上InDel0802和RM23432标记之间,目的基因与两个标记的遗传距离分别为0.58cM和1.3cM。据以上结果推测,该基因可能是一个调控水稻各轮花器官发育的新基因。

关键词: 水稻, 花发育, 突变体, fro1(t), 基因定位

Abstract: It's important to study the mechanisms of the rice floral development,because the rice floral organs provides the basis of the rice grain formation.We found a rice floral organ mutant from tissue culture mutation generations,called function of reproductive organ(fro1(t)).The floret shape of fro1(t) mutant is similar to that of tiny green capsicum with a couple of distorted lemma and palea,but the spekelets cannot bloom even though after heading;the glumes almost degenerated and lodicules are homeotically transformed into leaf-like organ;stamen had a distorted and enlarged filament,and the anter also showed a distorted surface yet without any pollen;the number of stamen is 2-6,and noticely some of that are homeotically transformed into pistil-like organ with stigmas-like structure on the top;the ovary showed a nomal development,but most of styles cause a fusion with 3-4 stigmas.By SEM we found that the gene regulates the determination of the floral meristem and specification of floral organ identity.We carried out fine mapping of the gene responsible for the mutated trait.The result showed that the mutated trait was located on the long arm of rice chromosome 8 between the marker InDel0802 and RM23432,with genetic distance of 0.58 cM and 1.3 cM respectively.All the above suggests that the FRO1(t) gene may be a novel gene which regulates all whorls of floral organs in rice.Study of FRO1(t) will support a key evidence to uncover the molecular mechanisms of the rice floral development.

Key words: Rice, floral development, mutant fro1(t), gene-mapping

中图分类号:

S511

陈在杰, 吴煜兵, 陈少游, 刘华清, 王锋. 水稻生殖发育突变体fro1(t)的形态发生及基因定位[J]. 福建农业学报, 2010, (6): 677-683.

CHEN Zai-jie, WU Yi-bing, CHEN Shao-you, LIU Hua-qing, WANG Feng. Morphogenesis and gene-mapping of a reproducetive development mutant fro1(t) in rice[J]. Fujian Journal of Agricultural Sciences, 2010, (6): 677-683.

参考文献

[1] COEN E S,MEYEROWITZ E M.The war of the whorls:genetic interactions controlling flower development[J].Nature,1991,353 (6339):31-37.

[2] ANGENENT G C,FRANKEN J,et al.A novel class of MADS box genes is involved in ovule development in petunia[J].Plant Cell,1995,7 (10):1569-1582.

[3] THEISSEN G,SAEDLER H.Plant biology:Floral quartets[J].Nature,2001,409 (6819):469-471.

[4] PELAZ S,et al.B and C floral organ identity functions require SEPALLATA MADS-box genes[J].Nature,2000,405 (6783):200-203.

[5] CLARK S E,WILLIAMS R W,MEYEROWITZ E M.The CLAVATA1 gene encodes a putative receptor kinase that controls shoot and floral meristem size in Arabidopsis[J].Cell,1997,89 (4):575-585.

[6] SCHOOF H,LENHARD M,HAECKER A,et al.The stem cell population of Arabidopsis shoot meristems in maintained by a regulatory loop between the CLAVATA and WUSCHEL genes[J].Cell,2000,100 (6):635-644.

[7] KYOZUKA J,KOBAYASH1 T,MORITA M,et al.Spatially and temporally regulated expression of rice MADS box genes with similarity to Arabidopsis class A,B and C genes[J].Plant Cell Physiol,2000,41 (6):710-718.

[8] MASAHIRO YANO,YUICHI KATAYOSE,MOTOYUKI ASHIKARI,et al.Hd1,a Major Photoperiod Sensitivity Quantitative Trait Locus in Rice,Is Closely Related to the Arabidopais Flowering Time Gene CONSTANS[J].Plant Cell,2000,12 (12):2473-2484.

[9] KOMATSU M,CHUJO A,NAGATO Yet al.FRIZZY PANICLE is required to prevent the formation of axillary meristems and to establish floral meristem identity in rice spikelets[J].Development,2003,130:3841-3850.

[10] DONG YEON Lee,JINWON LEE,SUNOK MOON,et al.The rice heterochronic gene SUPERNUMERARY BRACT regulates the transition from spikelet meristem to floral meristem[J].Plant J,2007,49 (1):64-78.

[11] N1LLA PELUCCHI,FABIO FORNARA,CRISTINA FAVALLI,et al.Comparative analysis of rice MADS-box genes expressed during flower development[J].Sex Plant Reprod,2002,15:113-122.

[12] LEE S,JEON J S,AN K,et al.Alteration of floral organ identity in rice through ectopic expression of OsMADS16[J].Plants,2003,217:904-911.

[13] NAGASAWA N,M1YOSHI M,SANO Y,et al.SUPERWOMAN1 and DROOPING LEAF genes control floral organ identity in rice[J].Development,2003,130:705-718.

[14] YAMAGUCHI T,NAGASAWA N,KAWASAKI S,et al.The YABBY gene DROOPING LEAF regulates carpel specification and midrib development in Oryza sativa[J].Plant Cell,2004,16:500-509.

[15] JEON J S,JANG S,LEE S,et al.Leafy hull sterile1 is a homeotic mutation in a rice MADS-box gene affecting rice flower development[J].Plant Cell,2000,12:871-889.

[16] AGRAWAL K G,ABE K,YAMAZAKI M,et al.Conservation of the E-function for floral organ identity in rice revealed by the analysis of tissue culture-induced loss-of function mutants of the OsMADS1 gene[J].Plant Molecular Biology,2005,59:125-135.

[17] PRASAD K,PARAMESWARAN S,VIJAYRAGHAVAN U.OsMADS1,a rice MADS-box factor,controls differentiation of specific cell types in the lamina and pales and is an early-acting regulator of inner floral organs[J].Plant J,2005,43:915-928.

[18] SUZAKI T,SATO M,ASHIKARI M,et al.The gene FLORAL ORGAN NUMBER1 regulates floral meristem size in rice and encodes a leucine-rich repeat receptor kinase orthologous to Arabidopsis CLAVATA1[J].Development,2004,131:5649-5657.

[19] CHU H,QIAN Q,LIANG W,et al.The FLORAL ORGAN NUMBER4 gene encoding a putative ortholog of Arabidopsis CLAVATA3 regulates apical meristem size in rice[J].Plant Physiol,2006,142:1039-1052.

[20] 段远霖,李维明,吴为人,等.水稻小穗分化调控基因fzp(t)的遗传分析和分子标记定位[J].中国科学C辑,2003,33(1):27-32.

[21] MURRAY M G,THOMPSON W F.Rapid isolation of highmolecular weight plant DNA[J].Nucleic Acids Research,1980,8 (19):4321-4325.

[22] MICHELMORE R W,PARAN I,KESSELI R V.Identification of markers linked to disease-resistance genes by hulked segregation analysis:a rapid method to detect markers in specific genomic regions by using segregation population[J].Proc Natl Acad Sci,1991,88:9828-9832.

[23] 毛毕刚,刘华清.等.两个水稻生殖器官突变体的形态特征和遗传分析[J].分子植物育种,2008,6(2):233-238.

[24] HONGFA LUO,YUNFENG LI,et al.Fine mapping of a pistilloid-stamen (PS) gene on the short arm of chromosome 1 in rice[J].Genome,2006,49:1066-1022.