爱玉子瘦果果胶酯酶的抽提条件及活性测定研究

福建农业学报 ›› 2007, Vol. ›› Issue (4): 410-413.

爱玉子瘦果果胶酯酶的抽提条件及活性测定研究

吴文珊^1,2, 谢清清³, 郑翠芳², 朱晓东², 林玮², 黄美丽²

1. 福建农林大学生命科学学院, 福建, 福州, 350002;
2. 福建师范大学生命科学学院, 福建, 福州, 350007;
3. 厦门大学生命科学学院, 福建, 厦门, 361005

收稿日期:2007-01-16 修回日期:2007-09-18 出版日期:2007-12-15 发布日期:2007-12-15
基金资助:
福建省教育厅基金资助项目(K04029);福建省科技厅重点资助项目(2007S0015)

Extracting condition and the activity admeasurement for pectinesterase in achenes of Ficus awkeotsang Makino

WU Wen-shan^1,2, XIE Qing-qing³, ZHENG Cui-fang², ZHU Xiao-dong², LIN Wei², HUANG Mei-li²

1. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
2. College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350007, China;
3. College of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China

Received:2007-01-16 Revised:2007-09-18 Online:2007-12-15 Published:2007-12-15

摘要/Abstract

摘要： 研究了爱玉子果胶酯酶的优化提取方法,并测定了不同条件下爱玉子果胶酯酶的活性及热稳定性.结果表明:爱玉子果胶酯酶抽提液中NaCl的最佳浓度是5%,最佳抽提温度是30℃,最佳抽提时间是2 h,最佳保存时间2 h;酶活测定时作用液中NaCl最适浓度范围为0.6%～1.0%,作用液的最适pH为7.0,温度30～50℃,爱玉子果胶酯酶的热稳定性良好.

关键词: 爱玉子瘦果, 果胶酯酶活性, 抽提条件

Abstract: A method of extraction and assay for the pectinesterase were investigated and proposed as follows: the best concentration of NaCl in extracting mixtrure is 5%,the best extracting temperature is 30℃,the best extracting time is 24 hours,the best conservating time is 24 hours,the best concentration of NaCl in mixture is 0.6% to 1.0%,and maintain the pH on 7.0.Between 30-50℃,the pectinesterase in achenes of Ficus awkeotsang has a good thermal stability.

Key words: Ficus awkeotsang Makino achenes, pectinesterase activity, extracting condition

中图分类号:

TS201.25

吴文珊, 谢清清, 郑翠芳, 朱晓东, 林玮, 黄美丽. 爱玉子瘦果果胶酯酶的抽提条件及活性测定研究[J]. 福建农业学报, 2007, (4): 410-413.

WU Wen-shan, XIE Qing-qing, ZHENG Cui-fang, ZHU Xiao-dong, LIN Wei, HUANG Mei-li. Extracting condition and the activity admeasurement for pectinesterase in achenes of Ficus awkeotsang Makino[J]. Fujian Journal of Agricultural Sciences, 2007, (4): 410-413.