应用氯化锂法小量提取质粒DNA

福建农业学报 ›› 2007, Vol. ›› Issue (4): 469-470.

• 研究简报 • 上一篇

应用氯化锂法小量提取质粒DNA

潘建菁¹, 张君诚², 卢艺萍¹, 张杭颖², 庄伟建¹

1. 福建农林大学作物科学学院, 福建, 福州, 350002;
2. 三明学院, 福建, 三明, 365000

收稿日期:2007-02-27 修回日期:2007-12-12 出版日期:2007-12-15 发布日期:2007-12-15
基金资助:
国家烟草专卖局资助项目(110200601011)

A new method—LiCl for extracting bacterium plasmid DNA

PAN Jian-jing¹, ZHANG Jun-cheng², LU Yi-ping¹, ZHANG Hang-ying², ZHUANG Wei-jian¹

1. College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
2. Sanming University, Sanming, Fujian 365000, China

Received:2007-02-27 Revised:2007-12-12 Online:2007-12-15 Published:2007-12-15

摘要/Abstract

摘要： 用改良的氯化锂方法小量提取质粒DNA.结果表明,该方法所提取的质粒DNA的质量与常规碱裂解法获得的质粒DNA基本一致,可以满足分子生物学基础实验的要求;同时该方法简化了试验步骤,质粒DNA提取时间缩短了24 min.

关键词: 质粒DNA, 氯化锂

Abstract: A new method to extract small amount of plasmid DNA with LiCl was developed.The results showed that the quality of plasmid DNA extracted by using this method was basically identical to that of the conventional alkalinelysis.The method satisfied the need for basic molecular biology experiments,and the procedure was simplified shortening the extracting time by 24 min.

Key words: Plasmid DNA, LiCl

中图分类号:

Q-33

潘建菁, 张君诚, 卢艺萍, 张杭颖, 庄伟建. 应用氯化锂法小量提取质粒DNA[J]. 福建农业学报, 2007, (4): 469-470.

PAN Jian-jing, ZHANG Jun-cheng, LU Yi-ping, ZHANG Hang-ying, ZHUANG Wei-jian. A new method—LiCl for extracting bacterium plasmid DNA[J]. Fujian Journal of Agricultural Sciences, 2007, (4): 469-470.

应用氯化锂法小量提取质粒DNA

A new method—LiCl for extracting bacterium plasmid DNA

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