福建农业学报 ›› 2019, Vol. 34 ›› Issue (7): 829-836.doi: 10.19303/j.issn.1008-0384.2019.07.012

• 植物保护 • 上一篇    下一篇

高产木质素降解酶菌株的筛选及其生物学特性研究

袁海华1, 张保1, 张颖2, 敖新宇1   

  1. 1. 西南林业大学生命科学院, 云南 昆明 650224;
    2. 西南林业大学生物多样性保护与利用学院, 云南 昆明 650224
  • 收稿日期:2019-03-15 修回日期:2019-06-22 出版日期:2019-07-20 发布日期:2019-10-14
  • 通讯作者: 敖新宇(1978-),男,硕士,副教授,研究方向:生物化学与分子生物学(E-mail:54700875@qq.com) E-mail:54700875@qq.com
  • 作者简介:袁海华(1994-),女,硕士研究生,研究方向:生物化学与分子生物学(E-mail:1137304061@qq.com)
  • 基金资助:
    国家自然科学基金项目(31560013)

A Study on Ligninolytic Enzyme-producing Fungi

YUAN Hai-hua1, ZHANG Bao1, ZHANG Ying2, AO Xin-yu1   

  1. 1. College of Life Science, Southwest Forestry University, Kunming, Yunnan 650224, China;
    2. College of Biodiversity Conservation and Utilization, Southwest Forestry University, Kunming, Yunnan 650224, China
  • Received:2019-03-15 Revised:2019-06-22 Online:2019-07-20 Published:2019-10-14

摘要: [目的] 获得高产木质素降解酶(LMLs,包括漆酶Lac、锰过氧化物酶MnP、木质素过氧化物酶LiP)的菌株,为木腐菌菌种资源的合理开发和应用提供一定理论依据。[方法] 对9株具有产LMLs能力的木腐菌进行产酶筛选,并对筛选出的菌株的生物学特性进行研究。[结果] 结果表明:在9株木腐菌中,产LMLs能力较高的菌株分别为LS136(Lac:0.18 U·L-1、LiP:109.68 U·L-1、MnP:140.38 U·L-1)、LJ485(Lac:136.99 U·L-1、LiP:9.53 U·L-1、MnP:72.05 U·L-1)和LJ496(Lac:163.39 U·L-1、LiP:9.50 U·L-1、MnP:74.36 U·L-1)。生物学特性研究结果显示3种菌株最适生长温度均为25℃左右,适宜的生长pH值在4~8。此外,3种菌株对0~8 mmol·L-1的重金属Cr3+、Pb2+以及0~4 mmol·L-1的Cu2+均有较强的耐受性。当Cr3+为1~4 mmol·L-1时,对LJ485和LJ496的生长具有一定的促进作用。[结论] 菌株LS136、LJ485和LJ496分别属于韧革菌属Stereum sp.彩绒革盖菌Trametes versicolor和小薄孔菌Antrodiella sp.,具有较强的产LMLs能力和一定的重金属耐受性,为降解木质素的菌株开发提供了菌种资源参考。

关键词: 木腐菌, 木质素降解酶, 生物学特性

Abstract: [Objective] To search for wood-rotting fungi that are highly effective in producing ligninolytic enzymes(LMLs), such asLac, MnP, and LiP.[Methods] Nine strains of wood-rotting fungi with known ability to produce LMLs were screened and their biological characteristics studied.[Results] The strains with high capacity of generating LMLs and their yields on each of the LMLs were found to be:LS136 (yielding Lac at 0.18 U·L-1, LiP at 109.68 U·L-1, and MnP at 140.38 U·L-1), LJ485 (yielding Lac at 136.99 U·L-1, LiP at 9.53 U·L-1, and MnP at 72.05 U·L-1), and LJ496 (yielding Lac at 163.39 U·L-1, LiP at 9.50 U·L-1, and MnP at 74.36 U·L-1). The optimum growth temperature for the 3 fungal strains was approximately 25℃, and pH ranged 4 to 8. The strains were all highly tolerant to Cr3+ or Pb2+ at concentrations of 0-8 mmoL·L-1 and Cu2+ at 0-4 mmoL·L-1. At the concentrations between 1-4 mmoL·L-1, Cr3+ promoted the growth of LJ485 and LJ496.[Conclusion] The identified LS136 was thought to be a Stereum sp., LJ485 identified as Trametes versicolor, and LJ496 considered an Antrodiella sp.. They all demonstrated varying capacities of producing LMLs with a tolerance to heavy metals. The information would be useful for further investigation on the utilization of these lignin degrading fungi.

Key words: wood-rotting fungus, ligninolytic enzymes, biological characteristics

中图分类号: 

  • Q93