稀酸提取羊栖菜多糖的结构及其抗氧化特性研究

doi:10.19303/j.issn.1008-0384.2019.07.014

摘要/Abstract

摘要： [目的] 详细探讨了不同醇沉条件制备的羊栖菜多糖组分的单糖组成、平均分子量、结构及其抗氧化特性。[方法] 通过稀酸提取法制备羊栖菜提取液，用不同浓度的乙醇沉淀制备羊栖菜多糖组分。利用柱前衍生-反相高效液相色谱、凝胶渗透色谱与多角度激光光散射联用、傅里叶变换红外光谱、紫外分光光度计、扫描电子显微镜以及哈克MARS Ⅲ型流变仪等技术手段，分析了多糖组分的基本成分、单糖组成、平均分子量、有机官能团、空间构象、表观形貌及流变学特性。此外，进一步研究了各多糖组分的氧自由基吸收能力以及DPPH·和ABTS·的清除能力。[结果] SFP-50和SFP-70具有高的总糖、硫酸基及糖醛酸含量；5种多糖组分均由甘露糖、葡萄糖、葡萄糖醛酸、半乳糖、阿拉伯糖和岩藻糖组成，但占比均不相同，其中岩藻糖占比都最高，约为50%；SFP-40、SFP-50、SFP-60、SFP-70和SFP-80的平均分子量分别为97.63、90.51、97.19、81.52及90.93 kDa；流变试验表明羊栖菜多糖溶液属于非牛顿流体，表现为固体弹性性质；扫描电镜图像揭示羊栖菜多糖组分表面呈凹凸不平、疏松多孔的褶皱结构；抗氧化特性分析结果表明羊栖菜多糖组分具有一定的氧自由基吸收能力以及DPPH·和ABTS·的清除能力，且不同体积分数乙醇醇沉会影响其抗氧化特性。[结论] 不同体积分数乙醇醇沉会影响羊栖菜多糖的理化性质、单糖组成和平均分子量，但不改变其构象、微观结构及流变学性质，对其抗氧化活性也有一定的影响。

关键词: 羊栖菜, 多糖, 结构表征, 抗氧化活性

Abstract: [Objective] The polysaccharides extracted from Sargassum fusiforme with different ethanol volume were studied.[Method] Polysaccharides were extracted from S.fusiforme using an acid solution, then precipitated with different volume fractions of ethanol. The resulting extract was analyzed for its physicochemical properties, monosaccharide composition, molecular weight, structural characteristics, functional group identification, spatial conformation, apparent morphology, and rheological properties using ultraviolet spectrophotometer, precolumn derivatization-reverse-phase high performance liquid chromatography, GPC-MALLs, FT-IR, SEM, and Huck MARS Ⅲ rheometer. The antioxidant activity of the extracts, including the oxygen radical absorption capacity and DPPH· and ABTS· scavenging ability, were determined.[Result] Extracts, SFP-50 and SFP-70, were found to contain higher contents of sugar, sulfate and uronic acid than the other samples. All 5 extracts contained mannose, glucose, glucuronic acid, galactose, arabinose, and fucose but differed in ratio, with fucose being the highest at about 50% of them combined. The molecular weights of SFP-40, SFP-50, SFP-60, SFP-70, and SFP-80 were estimated to be 97.63, 90.51, 97.19, 81.52, and 90.93 kDa, respectively. The Congo red assay showed that the polysaccharides had a triple helical structure. The solution of the extracts was non-Newtonian fluid with elasticity. The scanning electron microscopy images showed an uneven, loose and porous surface. All samples had varying degrees of oxygen radical absorption capacity and DPPH· and ABTS· scavenging ability.[Conclusion] The volume fraction employed for the ethanol precipitation affected the physicochemical properties, monosaccharide composition, and molecular weight as well as the antioxidant activity, but not the conformation, microstructure or rheological properties, of the polysaccharides obtained.

Key words: Sargassum fusiforme, polysaccharides, structural characterization, antioxidant activity

中图分类号:

R284.2

吴娟, 欧志荣, 李昭蓉, 赵谋明. 稀酸提取羊栖菜多糖的结构及其抗氧化特性研究[J]. 福建农业学报, 2019, 34(7): 842-851.

WU Juan, OU Zhi-rong, LI Zhao-rong, ZHAO Mou-ming. Structure and Antioxidant Activity of Polysaccharides Extracted from Sargassum fusiforme[J]. Fujian Journal of Agricultural Sciences, 2019, 34(7): 842-851.

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