酸铝对杉木幼苗叶片抗氧化酶活性的影响

doi:10.19303/j.issn.1008-0384.2019.07.015

福建农业学报 ›› 2019, Vol. 34 ›› Issue (7): 852-857.doi: 10.19303/j.issn.1008-0384.2019.07.015

酸铝对杉木幼苗叶片抗氧化酶活性的影响

王培^1,2, 张家君^1,2, 刘翠^1,2, 马志慧^2,3, 陈宇^1,2, 林思祖^1,2

1. 福建农林大学林学院, 福建福州 350002;
2. 国家林业局杉木工程技术研究中心, 福建福州 350002;
3. 福建农林大学生命科学学院, 福建福州 350002

收稿日期:2018-11-09 修回日期:2019-06-28 出版日期:2019-07-20 发布日期:2019-10-14
通讯作者: 陈宇(1983-),男,主要从事林木遗传育种研究(E-mail:28811852@qq.com) E-mail:28811852@qq.com
作者简介:王培(1993-),女,硕士研究生,主要从事林木遗传育种研究(E-mail:790646055@qq.com)
基金资助:
国家林业局杉木工程技术研究中心平台建设项目（ptjh130002）；国家林业局杉木工程技术研究中心孵化基金（6213C011103）

Physiological Response of Chinese Fir (Cunninghamia lanceolata) Seedlings Under Acid and/or Aluminum Stresses

WANG Pei^1,2, ZHANG Jia-jun^1,2, LIU Cui^1,2, MA Zhi-hui^2,3, CHEN Yu^1,2, LIN Si-zu^1,2

1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
2. State Forestry Administration Engineering Research Center of Chinese Fir, Fuzhou, Fujian 350002, China;
3. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

Received:2018-11-09 Revised:2019-06-28 Online:2019-07-20 Published:2019-10-14

摘要/Abstract

摘要： [目的] 为了探究杉木在酸铝胁迫下抗氧化酶活性的变化规律及其作用机理，以此为基础探索逆境胁迫下杉木的抗性机制。[方法] 通过土培盆栽试验，以1年生杉木实生苗为试验对象，采用0.24 g·kg^-1的AlCl₃·6H₂O模拟铝胁迫、pH 4.0的酸液模拟酸胁迫以及两者共施模拟酸铝复合胁迫，测定幼苗叶片不同胁迫时间（15、30和45 d）的MDA含量和SOD、POD、CAT、PPO等酶活性。[结果] 无酸无铝状态下，MDA含量均处于低水平状态，单酸、单铝和酸铝处理皆引发MDA的过量积累，且影响程度是酸铝复合胁迫 > 铝胁迫 > 酸胁迫。在单酸、单铝和酸铝处理下，发现POD和SOD酶活性的增长幅度是酸铝复合胁迫 > 酸胁迫 > 铝胁迫；CAT和PPO酶活性的增长幅度则是酸铝复合胁迫 > 铝胁迫 > 酸胁迫。随着胁迫时间延长，POD、SOD活性均先增后减；CAT活性在铝胁迫和酸铝复合胁迫下先增后减，酸胁迫下逐渐增加；PPO活性则均逐渐增加。[结论] 单酸、单铝和酸铝处理对POD、SOD、CAT和PPO活性均产生不同程度的诱导，但同时引发MDA的积累。酸铝复合胁迫对抗氧化酶活性具有一定的协同效应，酸胁迫对POD和SOD活性的促进作用大于铝胁迫，对于CAT和PPO活性，铝胁迫的促进作用则大于酸胁迫。

关键词: 杉木, 酸铝, 抗氧化酶活性, MDA

Abstract: [Objective] The antioxidant enzyme activities and respond mechanism of Chinese fir (Cunninghamia lanceolata) under acid and/or aluminum stresses were investigated.[Method] A pot experiment was conducted to determine the MDA content and SOD, POD, CAT, and PPO activities in one-year-old leaves of Chinese fir seedlings under the stress for 15, 30 or 45 d. At the rate of 0.24 g·kg^-1, AlCl₃ 6H₂O was applied to the pots to simulate an aluminum stress, a pH 4.0 acid solution added to simulate an acid stress, and the combination of the two incorporated to simulate an acid-aluminum stress.[Result] Without the presence of acid or aluminum, the potted seedlings had a low content of MDA in the leaves. However, the addition of acid, aluminum or acid-aluminum combination induced excessive accumulation of MDA with the severities ranking as acid-aluminum stress > aluminum stress > acid stress. The addition also raised the POD and SOD activities in leaves with a rank of acid-aluminum stress > acid stress > aluminum stress, and the CAT and PPO activities in the order of acid-aluminum stress > aluminum stress > acid stress. A prolonged treatment caused an initial increase on the POD and SOD activities followed by a decline; and on the CAT activity similarly under the aluminum or acid-aluminum stress but a steady increase under the acid stress; whereas, a gradual increase on the PPO activity under any of the 3 treatments.[Conclusion] The acid, aluminum or acid-aluminum stress affected the POD, SOD, CAT and PPO activities in the leaves to varying extents and raised MDA accumulation in the leaves of Chinese fir seedlings. There appeared to be a synergistic effect on the activities between acid and aluminum. But alone, the acid stress exerted a greater effect on POD and SOD than did aluminum, whereas, aluminum seemed to be a more formidable adversary than acid for the seedlings on CAT and PPO.

Key words: Chinese fir, acid-aluminum stress, oxidase activity, MDA

中图分类号:

Q945.78

王培, 张家君, 刘翠, 马志慧, 陈宇, 林思祖. 酸铝对杉木幼苗叶片抗氧化酶活性的影响[J]. 福建农业学报, 2019, 34(7): 852-857.

WANG Pei, ZHANG Jia-jun, LIU Cui, MA Zhi-hui, CHEN Yu, LIN Si-zu. Physiological Response of Chinese Fir (Cunninghamia lanceolata) Seedlings Under Acid and/or Aluminum Stresses[J]. Fujian Journal of Agricultural Sciences, 2019, 34(7): 852-857.

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酸铝对杉木幼苗叶片抗氧化酶活性的影响

Physiological Response of Chinese Fir (Cunninghamia lanceolata) Seedlings Under Acid and/or Aluminum Stresses

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